Recombinant DNA and transformant containing the same


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

Tetsuya Mori , Kozo Yamamoto , Tsunetaka Ohta

ABSTRACT

Insertion of IFN-alpha promoters in recombinant DNAs improves their expression efficiencies for useful polypeptides. Expression of such a recombinant DNA in host cells of mammalian origin is artificially controllable by the presence and absence of external stimuli using IFN-alpha inducers. Thus, transformants with such a recombinant DNA readily increase to a maximized cell density with causing neither damages nor extinction due to polypeptides they produce, and subsequent exposure to IFN-alpha inducers allows the proliferated cells to efficiently produce polypeptides with significant glycosylations. More... »

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