Expression of fusion polypeptides transported out of the cytoplasm without leader sequences


Ontology type: sgo:Patent     


Patent Info

DATE

1997-05-13T00:00

AUTHORS

Desmond Mascarenhas , Yang Zhang , Pamela S. Olson , David R. Olsen , Pedro A. Cohen

ABSTRACT

The invention is directed to the use of a fusion partner that does not contain a leader sequence, as a means to increase the solubility and activity of recombinant polypeptides by facilitating the expression of fusion proteins, which are then transported out of the cytoplasm. The invention includes a nucleic acid encoding a fusion polypeptide comprising a mature interleukin-1-like polypeptide or a leader-deleted-translocating polypeptide, and a polypeptide of interest; as well as host cells comprising such nucleic acids, and fusion proteins so encoded. The invention also encompasses methods of using such nucleic acids to produce recombinant fusion polypeptides, mature polypeptides of interest, and purified compositions thereof. More... »

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