Enzymes for the production of 2-keto-L-gulonic acid


Ontology type: sgo:Patent     


Patent Info

DATE

1996-12-10T00:00

AUTHORS

Robert A. Lazarus , Mark Hurle , Stephen Anderson , David B. Powers

ABSTRACT

Mutants of 2,5-diketo-D-gluconic acid reductase A, an enzyme used to produce 2-keto-L-gulonic acid, a precursor of ascorbic acid (vitamin C) are prepared by site-directed mutagenesis. These mutants have increased catalytic activity, increased expression levels, and/or enhanced temperature stability.

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