Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters


Ontology type: sgo:Patent     


Patent Info

DATE

1995-11-07T00:00

AUTHORS

GOSSEN MANFRED , BUJARD HERMANN

ABSTRACT

A first polynucleotide molecule coding for a transactivator fusion protein comprising the tet repressor and a protein capable of activating transcription in eucaryotes. A second polynucleotide molecule coding for a protein, wherein said polynucleotide is operably linked to a minimal promoter operably linked to at least one tet operator sequence is also disclosed. A method to regulate the expression of a protein coded for by a polynucleotide, by cultivating the eucaryotic cell of the invention in a medium comprising tetracycline or a tetracycline analogue is also disclosed. Kits containing the polynucleotide molecules are also disclosed. More... »

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