Animal Cell Transformant, Method For Obtaining The Transformant And Method For Producing Desired Foreign Gene Product By Using The Transformant


Ontology type: sgo:Patent     


Patent Info

DATE

1993-06-15T00:00

AUTHORS

Noboru Tomioka , Fumio Omae , Eriko Mine , Tomoko Ishii

ABSTRACT

By screening an animal cell transformant, which has been obtained by introducing into wild-type animal cells a wild-type DHFR gene and a structural gene which codes a desired useful substance, with a medium containing methotrexate (MTX) at a concentration of 50 μM or lower, both genes can be amplified effectively within an MTX resistant strain and a stable amplified transformant can also be obtained. The screening may be repeated using an increasing concentration of MTX. It is also possible to obtain stably the desired foreign gene product in a high yield by using the thus-obtained amplified transformant. More... »

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