Molecular Cloning And Expression Of Genes Encoding Proteolytic Enzymes


Ontology type: sgo:Patent     


Patent Info

DATE

1993-06-08T00:00

AUTHORS

Christiaan A. G. van Eekelen , Johannes C. van der Laan , Leo J. S. M. Mulleners

ABSTRACT

Novel methods and novel industrial Bacillus strains are provided for enhanced production of serine protease. Plasmid constructs comprising the serine protease gene are introduced into a compatible host, generally a Bacillus already overproducing serine protease. Preferred host cells are those from Bacillus novo sp. PB92 and the preferred serine protease gene also originates from Bacillus PB92. Integration and maintenance of the plasmid in the host cell chromosome can be achieved by including a temperature sensitive origin of replication in the plasmid construct and growing under selective conditions. More... »

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