Control of gene expression by glucose, calcium and temperature


Ontology type: sgo:Patent     


Patent Info

DATE

1993-03-23T00:00

AUTHORS

Amy S. Lee

ABSTRACT

The regulatory sequence for a glucose regulated protein has been isolated. When fused into suitable expression vectors within reading relationship of a desired polypeptide DNA sequence, the resulting hybrid gene can be induced to high levels by glucose starvation, or calcium shock, or by denaturing reagents such as β-mercaptoethanol, in a variety of host systems. Further, the hybrid gene can also be regulated by temperature when it is introduced into temperature-sensitive host systems such as the temperature-sensitive mutant of Chinese hamster cells or other systems having a compatible mutation. More... »

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