Complementary Dna Strands Covalently Bonded By A Phosphate Ester To Rna; Transforming Bacterial Cells To Produce Specified Polypeptides


Ontology type: sgo:Patent     


Patent Info

DATE

1989-05-16T00:00

AUTHORS

PADDOCK GARY V

ABSTRACT

Compounds useful as complementary DNA (cDNA) include deoxyribonucleotides and at least one ribonucleotide. They may be depicted by the general formula: <IMAGE> wherein (dN)a and (dN)c represent series of deoxyribonucleotides and (rN)b represents a series of ribonucleotides; wherein a, b, and c are the number of nucleotides in the series, with the proviso that b is >/=1, a is >/=35, and c is >/=10; wherein the series of deoxyribonucleotides (dN)a includes a series of deoxyribonucleotides which is substantially complementary to the series of deoxyribonucleotides (dN)c and the dashed line represents non-covalent bonding between the complementary deoxyribonucleotide series; and wherein the solid line represents a covalent phosphodiester bond. These compounds may be prepared from messenger RNA (mRNA) containing the genetic information necessary for cellular production of desired products such as polypeptides. After appropriate modification, they may be combined with DNA from a suitable cloning vehicle such as a plasmid and the resulting combined DNA used to transform bacterial cells. The transformed bacterial cells may then be grown and harvested; and the desired product or products recovered. More... »

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