Methods and compositions for targeted genetic modification using paired guide RNAs


Ontology type: sgo:Patent     


Patent Info

DATE

2019-10-29T00:00

AUTHORS

FRENDEWEY DAVID , LAI KA-MAN VENUS , AUERBACH WOJTEK , DROGUETT GUSTAVO , GAGLIARDI ANTHONY , VALENZUELA DAVID M , VORONINA VERA , MACDONALD LYNN , MURPHY ANDREW J , YANCOPOULOS GEORGE D

ABSTRACT

Compositions and methods are provided for creating and promoting biallelic targeted modifications to genomes within cells and for producing non-human animals comprising the modified genomes. Also provided are compositions and methods for modifying a genome within a cell that is heterozygous for an allele to become homozygous for that allele. The methods make use of Cas proteins and two or more guide RNAs that target different locations within the same genomic target locus. Also provided are methods of identifying cells with modified genomes. More... »

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