Methods and compositions for the targeted modification of a mouse embryonic stem cell genome


Ontology type: sgo:Patent     


Patent Info

DATE

2019-02-19T00:00

AUTHORS

FRENDEWEY DAVID , AUERBACH WOJTEK , LAI KA-MAN VENUS , VALENZUELA DAVID M , YANCOPOULOS GEORGE D

ABSTRACT

Compositions and methods are provided for modifying a genomic locus of interest in a eukaryotic cell, a mammalian cell, a human cell or a non-human mammalian cell using a large targeting vector (LTVEC) comprising various endogenous or exogenous nucleic acid sequences as described herein. Further methods combine the use of the LTVEC with a CRISPR/Cas system. Compositions and methods for generating a genetically modified non-human animal comprising one or more targeted genetic modifications in their germline are also provided. More... »

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