Deconstructing overlapped peaks in droplet digital polymerase chain reaction data


Ontology type: sgo:Patent     


Patent Info

DATE

2018-11-13T00:00

AUTHORS

LERNER JEFFREY

ABSTRACT

To determine a peak signal corresponding to a particular part of a sample, a data signal for a cluster can be transformed into a set of localized functions. For example, the data signal can be transformed to obtain coefficients of a set of wavelets, which can span a variety of scales that define an exponential decay of the wavelet. The coefficients in a time region for which a peak signal is to be obtained can be replaced with coefficients that model the behavior of tails of other peaks. The tail model can be determined using nonlinear regression, which may need to only be performed once. An inverse transform can then provide a background signal that can be subtracted from the input data signal to provide the desired peak signal. More... »

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