Amplification of bisulfite-reacted nucleic acids


Ontology type: sgo:Patent     


Patent Info

DATE

2018-10-02T00:00

AUTHORS

Christian Korfhage , Dirk Loeffert , Ralf Peist , Nicolas Rudinger

ABSTRACT

The present invention relates to a reaction mixture for the amplification of nucleic acids, the non-methylated cytosine bases of which have been converted to uracil bases by means of a bisulfition reaction. The invention also discloses methods for amplifying bisulfited nucleic acid and for determining the nucleic acid methylation state, and also kits based on the reaction mixture according to the invention. More... »

Related SciGraph Publications

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2447", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "name": "Christian Korfhage", 
        "type": "Person"
      }, 
      {
        "name": "Dirk Loeffert", 
        "type": "Person"
      }, 
      {
        "name": "Ralf Peist", 
        "type": "Person"
      }, 
      {
        "name": "Nicolas Rudinger", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.jviromet.2008.01.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015003693"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jcb.10740", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015485947"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jcv.2006.02.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034272567"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.082089499", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042072026"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.compbiolchem.2008.02.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050561691"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1205996", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051404061", 
          "https://doi.org/10.1038/sj.onc.1205996"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.onc.1205996", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051404061", 
          "https://doi.org/10.1038/sj.onc.1205996"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2144/000112266", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069095387"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-10-02T00:00", 
    "description": "

The present invention relates to a reaction mixture for the amplification of nucleic acids, the non-methylated cytosine bases of which have been converted to uracil bases by means of a bisulfition reaction. The invention also discloses methods for amplifying bisulfited nucleic acid and for determining the nucleic acid methylation state, and also kits based on the reaction mixture according to the invention.

", "id": "sg:patent.US-10087482-B2", "keywords": [ "amplification", "nucleic acid", "invention", "reaction mixture", "methylated cytosine", "uracil", "mean", "reaction", "method", "methylation state", "kit" ], "name": "Amplification of bisulfite-reacted nucleic acids", "recipient": [ { "id": "https://www.grid.ac/institutes/grid.420167.6", "type": "Organization" } ], "sameAs": [ "https://app.dimensions.ai/details/patent/US-10087482-B2" ], "sdDataset": "patents", "sdDatePublished": "2019-04-18T10:23", "sdLicense": "https://scigraph.springernature.com/explorer/license/", "sdPublisher": { "name": "Springer Nature - SN SciGraph project", "type": "Organization" }, "sdSource": "s3://com-uberresearch-data-patents-target-20190320-rc/data/sn-export/402f166718b70575fb5d4ffe01f064d1/0000100128-0000352499/json_export_01845.jsonl", "type": "Patent" } ]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/patent.US-10087482-B2'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/patent.US-10087482-B2'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/patent.US-10087482-B2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/patent.US-10087482-B2'


 

This table displays all metadata directly associated to this object as RDF triples.

67 TRIPLES      15 PREDICATES      32 URIs      19 LITERALS      2 BLANK NODES

Subject Predicate Object
1 sg:patent.US-10087482-B2 schema:about anzsrc-for:2447
2 schema:author N94ffd89b16a84d858a33d12dff9d2596
3 schema:citation sg:pub.10.1038/sj.onc.1205996
4 https://doi.org/10.1002/jcb.10740
5 https://doi.org/10.1016/j.compbiolchem.2008.02.004
6 https://doi.org/10.1016/j.jcv.2006.02.008
7 https://doi.org/10.1016/j.jviromet.2008.01.022
8 https://doi.org/10.1073/pnas.082089499
9 https://doi.org/10.2144/000112266
10 schema:datePublished 2018-10-02T00:00
11 schema:description <p>The present invention relates to a reaction mixture for the amplification of nucleic acids, the non-methylated cytosine bases of which have been converted to uracil bases by means of a bisulfition reaction. The invention also discloses methods for amplifying bisulfited nucleic acid and for determining the nucleic acid methylation state, and also kits based on the reaction mixture according to the invention.</p>
12 schema:keywords amplification
13 invention
14 kit
15 mean
16 method
17 methylated cytosine
18 methylation state
19 nucleic acid
20 reaction
21 reaction mixture
22 uracil
23 schema:name Amplification of bisulfite-reacted nucleic acids
24 schema:recipient https://www.grid.ac/institutes/grid.420167.6
25 schema:sameAs https://app.dimensions.ai/details/patent/US-10087482-B2
26 schema:sdDatePublished 2019-04-18T10:23
27 schema:sdLicense https://scigraph.springernature.com/explorer/license/
28 schema:sdPublisher Na83af372486a4d0bae91ee4fecf21918
29 sgo:license sg:explorer/license/
30 sgo:sdDataset patents
31 rdf:type sgo:Patent
32 N14baa6b53ac54b01bddb8b09064c9a75 rdf:first Na16a6f8909de4286b7673b154bdda687
33 rdf:rest rdf:nil
34 N35a58b83a0e849c9b9c0892cb42a2eff rdf:first Nce4570c19e4f4da38773ba23c4bd4720
35 rdf:rest N14baa6b53ac54b01bddb8b09064c9a75
36 N94ffd89b16a84d858a33d12dff9d2596 rdf:first Nc76c7c9b4bb34f12ab542b355fd587ff
37 rdf:rest N9a137562b4d449bd91e6e4cb9692b741
38 N9a137562b4d449bd91e6e4cb9692b741 rdf:first Nbf45ca60c9994aad862da71de18a310e
39 rdf:rest N35a58b83a0e849c9b9c0892cb42a2eff
40 Na16a6f8909de4286b7673b154bdda687 schema:name Nicolas Rudinger
41 rdf:type schema:Person
42 Na83af372486a4d0bae91ee4fecf21918 schema:name Springer Nature - SN SciGraph project
43 rdf:type schema:Organization
44 Nbf45ca60c9994aad862da71de18a310e schema:name Dirk Loeffert
45 rdf:type schema:Person
46 Nc76c7c9b4bb34f12ab542b355fd587ff schema:name Christian Korfhage
47 rdf:type schema:Person
48 Nce4570c19e4f4da38773ba23c4bd4720 schema:name Ralf Peist
49 rdf:type schema:Person
50 anzsrc-for:2447 schema:inDefinedTermSet anzsrc-for:
51 rdf:type schema:DefinedTerm
52 sg:pub.10.1038/sj.onc.1205996 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051404061
53 https://doi.org/10.1038/sj.onc.1205996
54 rdf:type schema:CreativeWork
55 https://doi.org/10.1002/jcb.10740 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015485947
56 rdf:type schema:CreativeWork
57 https://doi.org/10.1016/j.compbiolchem.2008.02.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050561691
58 rdf:type schema:CreativeWork
59 https://doi.org/10.1016/j.jcv.2006.02.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034272567
60 rdf:type schema:CreativeWork
61 https://doi.org/10.1016/j.jviromet.2008.01.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015003693
62 rdf:type schema:CreativeWork
63 https://doi.org/10.1073/pnas.082089499 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042072026
64 rdf:type schema:CreativeWork
65 https://doi.org/10.2144/000112266 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069095387
66 rdf:type schema:CreativeWork
67 https://www.grid.ac/institutes/grid.420167.6 schema:Organization
 




Preview window. Press ESC to close (or click here)


...