High throughput detection of molecular markers based on AFLP and high through-put sequencing


Ontology type: sgo:Patent     


Patent Info

DATE

2018-07-17T00:00

AUTHORS

Michael Josephus Theresia Van Eijk , Rene Cornelis Josephus Hogers

ABSTRACT

The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3′ end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular marker. More... »

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