Use Of Brown Midrib-3 Gene Specific Markers In Corn For Trait Introgression


Ontology type: sgo:Patent     


Patent Info

DATE

2017-01-26T00:00

AUTHORS

WEI ZHENG , NATHAN VAN OPDORP , CHANDRA-SHEKARA CHANNABASAVARADHYA , SIVA P KUMPATLA

ABSTRACT

PROBLEM TO BE SOLVED: To provide compositions and methods for determining the zygosity of corn plants containing one or more brown midrib (BMR) mutations, as well as to provide methods that are useful for enhancing the breeding process for BMR corn.SOLUTION: The present invention relates to a method for determining the zygosity and/or presence/absence of an allele using corn plant tissue, the method comprising: obtaining a sample of isolated genomic DNA from the corn plant tissue; contacting with at least one nucleic acid molecule having at least 18 nucleic acid length comprising a nucleic acid sequence which can hybridize in a particular region of a polynucleotide of a specific sequence; and determining zygosity of a bm3 mutation in the isolated genomic DNA.SELECTED DRAWING: None More... »

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PROBLEM TO BE SOLVED: To provide compositions and methods for determining the zygosity of corn plants containing one or more brown midrib (BMR) mutations, as well as to provide methods that are useful for enhancing the breeding process for BMR corn.SOLUTION: The present invention relates to a method for determining the zygosity and/or presence/absence of an allele using corn plant tissue, the method comprising: obtaining a sample of isolated genomic DNA from the corn plant tissue; contacting with at least one nucleic acid molecule having at least 18 nucleic acid length comprising a nucleic acid sequence which can hybridize in a particular region of a polynucleotide of a specific sequence; and determining zygosity of a bm3 mutation in the isolated genomic DNA.SELECTED DRAWING: None

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