Methods for increasing Cas9-mediated engineering efficiency


Ontology type: sgo:Patent     


Patent Info

DATE

2017-05-03T00:00

AUTHORS

Peter Sean Cameron , RACHEL E HAURWITZ , ANDREW P MAY , CHRISTOPHER H NYE , Megan Van Overbeek

ABSTRACT

Methods for use with Type II CRISPR-Cas9 systems for increasing Cas9-mediated genome engineering efficiency are disclosed. The methods can be used to decrease the number of off-target nucleic acid double-stranded breaks and/or to enhance homology-directed repair of a cleaved target nucleic acid.

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2620", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "name": "Peter Sean Cameron", 
        "type": "Person"
      }, 
      {
        "name": "RACHEL E HAURWITZ", 
        "type": "Person"
      }, 
      {
        "name": "ANDREW P MAY", 
        "type": "Person"
      }, 
      {
        "name": "CHRISTOPHER H NYE", 
        "type": "Person"
      }, 
      {
        "name": "Megan Van Overbeek", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.cell.2013.02.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008291683"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2013.02.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008291683"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2013.02.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008291683"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.2908", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012868720", 
          "https://doi.org/10.1038/nbt.2908"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1231143", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019873131"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.2909", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053226125", 
          "https://doi.org/10.1038/nbt.2909"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-05-03T00:00", 
    "description": "

Methods for use with Type II CRISPR-Cas9 systems for increasing Cas9-mediated genome engineering efficiency are disclosed. The methods can be used to decrease the number of off-target nucleic acid double-stranded breaks and/or to enhance homology-directed repair of a cleaved target nucleic acid.

", "id": "sg:patent.GB-2544001-A", "keywords": [ "method", "efficiency", "type II", "genome", "decrease", "double-stranded break", "homology-directed repair", "target nucleic acid" ], "name": "Methods for increasing Cas9-mediated engineering efficiency", "recipient": [ { "id": "https://www.grid.ac/institutes/grid.487961.5", "type": "Organization" } ], "sameAs": [ "https://app.dimensions.ai/details/patent/GB-2544001-A" ], "sdDataset": "patents", "sdDatePublished": "2019-04-18T10:19", "sdLicense": "https://scigraph.springernature.com/explorer/license/", "sdPublisher": { "name": "Springer Nature - SN SciGraph project", "type": "Organization" }, "sdSource": "s3://com-uberresearch-data-patents-target-20190320-rc/data/sn-export/402f166718b70575fb5d4ffe01f064d1/0000100128-0000352499/json_export_01458.jsonl", "type": "Patent" } ]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/patent.GB-2544001-A'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/patent.GB-2544001-A'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/patent.GB-2544001-A'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/patent.GB-2544001-A'


 

This table displays all metadata directly associated to this object as RDF triples.

60 TRIPLES      15 PREDICATES      26 URIs      16 LITERALS      2 BLANK NODES

Subject Predicate Object
1 sg:patent.GB-2544001-A schema:about anzsrc-for:2620
2 schema:author N318d165e271c4b57a8ee725845d74358
3 schema:citation sg:pub.10.1038/nbt.2908
4 sg:pub.10.1038/nbt.2909
5 https://doi.org/10.1016/j.cell.2013.02.022
6 https://doi.org/10.1126/science.1231143
7 schema:datePublished 2017-05-03T00:00
8 schema:description <p>Methods for use with Type II CRISPR-Cas9 systems for increasing Cas9-mediated genome engineering efficiency are disclosed. The methods can be used to decrease the number of off-target nucleic acid double-stranded breaks and/or to enhance homology-directed repair of a cleaved target nucleic acid.</p>
9 schema:keywords decrease
10 double-stranded break
11 efficiency
12 genome
13 homology-directed repair
14 method
15 target nucleic acid
16 type II
17 schema:name Methods for increasing Cas9-mediated engineering efficiency
18 schema:recipient https://www.grid.ac/institutes/grid.487961.5
19 schema:sameAs https://app.dimensions.ai/details/patent/GB-2544001-A
20 schema:sdDatePublished 2019-04-18T10:19
21 schema:sdLicense https://scigraph.springernature.com/explorer/license/
22 schema:sdPublisher N61447e7e64d04ce4b225d052e76301ce
23 sgo:license sg:explorer/license/
24 sgo:sdDataset patents
25 rdf:type sgo:Patent
26 N07a91599b1ab4eec865263ffe7f0fe65 schema:name RACHEL E HAURWITZ
27 rdf:type schema:Person
28 N318d165e271c4b57a8ee725845d74358 rdf:first N561ddfdd93ec49d593156fae05108095
29 rdf:rest Nf73e74c00d874c8682f2af556949d726
30 N561ddfdd93ec49d593156fae05108095 schema:name Peter Sean Cameron
31 rdf:type schema:Person
32 N61447e7e64d04ce4b225d052e76301ce schema:name Springer Nature - SN SciGraph project
33 rdf:type schema:Organization
34 N6e044c03993c4fb38e1f806e5747870e rdf:first Nd75b9a21956a4106889257522ddc3967
35 rdf:rest N7c96b5802c7a442ebd4a7da13d0ad11f
36 N7c96b5802c7a442ebd4a7da13d0ad11f rdf:first Nfcb704665c784d07a25ece77f7be99e5
37 rdf:rest rdf:nil
38 N7e965378c5df4e19bb5420bb9de66cfb rdf:first Nd2eb2a4925a34021aedd213b740443f7
39 rdf:rest N6e044c03993c4fb38e1f806e5747870e
40 Nd2eb2a4925a34021aedd213b740443f7 schema:name ANDREW P MAY
41 rdf:type schema:Person
42 Nd75b9a21956a4106889257522ddc3967 schema:name CHRISTOPHER H NYE
43 rdf:type schema:Person
44 Nf73e74c00d874c8682f2af556949d726 rdf:first N07a91599b1ab4eec865263ffe7f0fe65
45 rdf:rest N7e965378c5df4e19bb5420bb9de66cfb
46 Nfcb704665c784d07a25ece77f7be99e5 schema:name Megan Van Overbeek
47 rdf:type schema:Person
48 anzsrc-for:2620 schema:inDefinedTermSet anzsrc-for:
49 rdf:type schema:DefinedTerm
50 sg:pub.10.1038/nbt.2908 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012868720
51 https://doi.org/10.1038/nbt.2908
52 rdf:type schema:CreativeWork
53 sg:pub.10.1038/nbt.2909 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053226125
54 https://doi.org/10.1038/nbt.2909
55 rdf:type schema:CreativeWork
56 https://doi.org/10.1016/j.cell.2013.02.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008291683
57 rdf:type schema:CreativeWork
58 https://doi.org/10.1126/science.1231143 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019873131
59 rdf:type schema:CreativeWork
60 https://www.grid.ac/institutes/grid.487961.5 schema:Organization
 




Preview window. Press ESC to close (or click here)


...