Enrichment Of Dna Sequencing Libraries From Samples Containing Small Amounts Of Target Dna


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

BUSTAMANTE, Carlos D. , CARPENTER, Meredith L. , BUENROSTRO, Jason D. , GREENLEAF, William J.

ABSTRACT

Provided herein is a method for capturing DNA molecules in solution. The method may comprise: extracting DNA from a sample that comprises endogenous DNA and environmental DNA to produce extracted DNA; ligating universal adaptors to the extracted DNA; hybridizing the extracted DNA, in solution, with affinity-tagged RNA probes generated by: in vitro transcribing a library of fragmented reference genomic DNA that has been ligated to an RNA promoter adaptor, in the presence of an affinity-tagged ribonucleotide; binding the product with a capture agent that is tethered to a substrate in the presence of RNA oligonucleotides that are complementary to the adaptors, thereby capturing the hybridized DNA molecules on the substrate; washing the substrate to remove any unbound DNA molecules; and releasing the captured DNA molecules. A kit for performing the method is also provided. More... »

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