Method for semi-synthetic production of extremely pure minicircle DNA vectors from plasmids


Ontology type: sgo:Patent     


Patent Info

DATE

2012-04-11T00:00

AUTHORS

REHBERGER, BERND , Heine, Markus , WODARCZYK, CLAAS , WAGNER, ROLAND

ABSTRACT

Producing circular DNA-vectors (c-DNA-v) in super-helical form comprises: (a) splitting parental plasmids with restriction enzymes to obtain linear DNA vector fragments with the sequence of the DNA vector; (b) separating the linear DNA vector fragments from other products of the splitting reaction; (c) ligating the linear DNA vector fragment to obtain c-DNA-v in relaxed form; (d) separating the c-DNA-v from other products of the ligation; (e) twisting the c-DNA-v of step (d) with a gyrase to obtain c-DNA-v in super-helical form; and (f) optionally purifying the c-DNA-v in super-helical form. Producing circular DNA-vectors (c-DNA-v) in super-helical form comprises: (a) splitting parental plasmids with one or more restriction enzymes to obtain linear DNA vector fragments with the sequence of the DNA vector, where the parental plasmid contains the sequence of the DNA-vectors and heterologous sequences; (b) separating the linear DNA vector fragments from other products of the splitting reaction; (c) ligating the linear DNA vector fragment to obtain c-DNA-v in relaxed form; (d) separating the c-DNA-v from other products of the ligation; (e) twisting the c-DNA-v of step (d) with a gyrase to obtain c-DNA-v in super-helical form; and (f) optionally purifying the c-DNA-v in super-helical form for the separation of byproducts. Independent claims are included for: (1) a reagent kit for producing a circular DNA vector in super-helical form, comprising a ligase, a gyrase, and optionally one or more restriction enzymes; (2) preparation of a minicircle DNA vector in super-helical form, characterized by the absence of byproducts after PCR, preferably linear or circular miniplasmid and/or parental plasmid; and (3) a method for producing super-helical minicircle DNA vectors without the use of location (sequence) specific recombinases such as flippase recombination enzyme. More... »

Related SciGraph Publications

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2471", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "name": "REHBERGER, BERND", 
        "type": "Person"
      }, 
      {
        "name": "Heine, Markus", 
        "type": "Person"
      }, 
      {
        "name": "WODARCZYK, CLAAS", 
        "type": "Person"
      }, 
      {
        "name": "WAGNER, ROLAND", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/s1525-0016(03)00168-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003365119"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1525-0016(03)00168-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003365119"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.83.6.1641", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011028028"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3302231", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015932804", 
          "https://doi.org/10.1038/sj.gt.3302231"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3302231", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015932804", 
          "https://doi.org/10.1038/sj.gt.3302231"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jgm.1243", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025964723"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/15.13.5105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027547740"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.81.13.4046", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036391437"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jmb.2009.11.066", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047616121"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2012-04-11T00:00", 
    "description": "

Producing circular DNA-vectors (c-DNA-v) in super-helical form comprises: (a) splitting parental plasmids with restriction enzymes to obtain linear DNA vector fragments with the sequence of the DNA vector; (b) separating the linear DNA vector fragments from other products of the splitting reaction; (c) ligating the linear DNA vector fragment to obtain c-DNA-v in relaxed form; (d) separating the c-DNA-v from other products of the ligation; (e) twisting the c-DNA-v of step (d) with a gyrase to obtain c-DNA-v in super-helical form; and (f) optionally purifying the c-DNA-v in super-helical form. Producing circular DNA-vectors (c-DNA-v) in super-helical form comprises: (a) splitting parental plasmids with one or more restriction enzymes to obtain linear DNA vector fragments with the sequence of the DNA vector, where the parental plasmid contains the sequence of the DNA-vectors and heterologous sequences; (b) separating the linear DNA vector fragments from other products of the splitting reaction; (c) ligating the linear DNA vector fragment to obtain c-DNA-v in relaxed form; (d) separating the c-DNA-v from other products of the ligation; (e) twisting the c-DNA-v of step (d) with a gyrase to obtain c-DNA-v in super-helical form; and (f) optionally purifying the c-DNA-v in super-helical form for the separation of byproducts. Independent claims are included for: (1) a reagent kit for producing a circular DNA vector in super-helical form, comprising a ligase, a gyrase, and optionally one or more restriction enzymes; (2) preparation of a minicircle DNA vector in super-helical form, characterized by the absence of byproducts after PCR, preferably linear or circular miniplasmid and/or parental plasmid; and (3) a method for producing super-helical minicircle DNA vectors without the use of location (sequence) specific recombinases such as flippase recombination enzyme.

", "id": "sg:patent.EP-2439276-A1", "keywords": [ "method", "DNA vector", "plasmid", "circular DNA", "splitting", "restriction enzyme", "linear DNA", "sequence", "product", "ligation", "gyrase", "Divorce", "byproduct", "claim", "reagent kit", "Ligases", "preparation", "absence", "polymerase chain reaction", "location", "recombinase", "flippases" ], "name": "Method for semi-synthetic production of extremely pure minicircle DNA vectors from plasmids", "sameAs": [ "https://app.dimensions.ai/details/patent/EP-2439276-A1" ], "sdDataset": "patents", "sdDatePublished": "2019-04-18T10:08", "sdLicense": "https://scigraph.springernature.com/explorer/license/", "sdPublisher": { "name": "Springer Nature - SN SciGraph project", "type": "Organization" }, "sdSource": "s3://com-uberresearch-data-patents-target-20190320-rc/data/sn-export/402f166718b70575fb5d4ffe01f064d1/0000100128-0000352499/json_export_00333.jsonl", "type": "Patent" } ]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/patent.EP-2439276-A1'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/patent.EP-2439276-A1'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/patent.EP-2439276-A1'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/patent.EP-2439276-A1'


 

This table displays all metadata directly associated to this object as RDF triples.

76 TRIPLES      14 PREDICATES      42 URIs      30 LITERALS      2 BLANK NODES

Subject Predicate Object
1 sg:patent.EP-2439276-A1 schema:about anzsrc-for:2471
2 schema:author N982dbf81a1fb4193bbb2b74cb57abaf8
3 schema:citation sg:pub.10.1038/sj.gt.3302231
4 https://doi.org/10.1002/jgm.1243
5 https://doi.org/10.1016/j.jmb.2009.11.066
6 https://doi.org/10.1016/s1525-0016(03)00168-0
7 https://doi.org/10.1073/pnas.81.13.4046
8 https://doi.org/10.1073/pnas.83.6.1641
9 https://doi.org/10.1093/nar/15.13.5105
10 schema:datePublished 2012-04-11T00:00
11 schema:description <p>Producing circular DNA-vectors (c-DNA-v) in super-helical form comprises: (a) splitting parental plasmids with restriction enzymes to obtain linear DNA vector fragments with the sequence of the DNA vector; (b) separating the linear DNA vector fragments from other products of the splitting reaction; (c) ligating the linear DNA vector fragment to obtain c-DNA-v in relaxed form; (d) separating the c-DNA-v from other products of the ligation; (e) twisting the c-DNA-v of step (d) with a gyrase to obtain c-DNA-v in super-helical form; and (f) optionally purifying the c-DNA-v in super-helical form. Producing circular DNA-vectors (c-DNA-v) in super-helical form comprises: (a) splitting parental plasmids with one or more restriction enzymes to obtain linear DNA vector fragments with the sequence of the DNA vector, where the parental plasmid contains the sequence of the DNA-vectors and heterologous sequences; (b) separating the linear DNA vector fragments from other products of the splitting reaction; (c) ligating the linear DNA vector fragment to obtain c-DNA-v in relaxed form; (d) separating the c-DNA-v from other products of the ligation; (e) twisting the c-DNA-v of step (d) with a gyrase to obtain c-DNA-v in super-helical form; and (f) optionally purifying the c-DNA-v in super-helical form for the separation of byproducts. Independent claims are included for: (1) a reagent kit for producing a circular DNA vector in super-helical form, comprising a ligase, a gyrase, and optionally one or more restriction enzymes; (2) preparation of a minicircle DNA vector in super-helical form, characterized by the absence of byproducts after PCR, preferably linear or circular miniplasmid and/or parental plasmid; and (3) a method for producing super-helical minicircle DNA vectors without the use of location (sequence) specific recombinases such as flippase recombination enzyme.</p>
12 schema:keywords DNA vector
13 Divorce
14 Ligases
15 absence
16 byproduct
17 circular DNA
18 claim
19 flippases
20 gyrase
21 ligation
22 linear DNA
23 location
24 method
25 plasmid
26 polymerase chain reaction
27 preparation
28 product
29 reagent kit
30 recombinase
31 restriction enzyme
32 sequence
33 splitting
34 schema:name Method for semi-synthetic production of extremely pure minicircle DNA vectors from plasmids
35 schema:sameAs https://app.dimensions.ai/details/patent/EP-2439276-A1
36 schema:sdDatePublished 2019-04-18T10:08
37 schema:sdLicense https://scigraph.springernature.com/explorer/license/
38 schema:sdPublisher Nc97a87764fb24b489641a9d2062545e0
39 sgo:license sg:explorer/license/
40 sgo:sdDataset patents
41 rdf:type sgo:Patent
42 N0e4c887584f94812a17efb4ca04e02ed schema:name WAGNER, ROLAND
43 rdf:type schema:Person
44 N5114e32819e745c988667e217ecf8701 rdf:first N7f8041d346f849a6adef65f3adbb63b4
45 rdf:rest N74db52f7f0eb4c45b2aff3dcf527af89
46 N74db52f7f0eb4c45b2aff3dcf527af89 rdf:first N0e4c887584f94812a17efb4ca04e02ed
47 rdf:rest rdf:nil
48 N7f8041d346f849a6adef65f3adbb63b4 schema:name WODARCZYK, CLAAS
49 rdf:type schema:Person
50 N982dbf81a1fb4193bbb2b74cb57abaf8 rdf:first Nfff65b7dd26349279707c965da395430
51 rdf:rest N991d424f69254afcb1e5b047b85158f3
52 N991d424f69254afcb1e5b047b85158f3 rdf:first Nac8e337bd6aa432ba129e9fae232dff4
53 rdf:rest N5114e32819e745c988667e217ecf8701
54 Nac8e337bd6aa432ba129e9fae232dff4 schema:name Heine, Markus
55 rdf:type schema:Person
56 Nc97a87764fb24b489641a9d2062545e0 schema:name Springer Nature - SN SciGraph project
57 rdf:type schema:Organization
58 Nfff65b7dd26349279707c965da395430 schema:name REHBERGER, BERND
59 rdf:type schema:Person
60 anzsrc-for:2471 schema:inDefinedTermSet anzsrc-for:
61 rdf:type schema:DefinedTerm
62 sg:pub.10.1038/sj.gt.3302231 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015932804
63 https://doi.org/10.1038/sj.gt.3302231
64 rdf:type schema:CreativeWork
65 https://doi.org/10.1002/jgm.1243 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025964723
66 rdf:type schema:CreativeWork
67 https://doi.org/10.1016/j.jmb.2009.11.066 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047616121
68 rdf:type schema:CreativeWork
69 https://doi.org/10.1016/s1525-0016(03)00168-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003365119
70 rdf:type schema:CreativeWork
71 https://doi.org/10.1073/pnas.81.13.4046 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036391437
72 rdf:type schema:CreativeWork
73 https://doi.org/10.1073/pnas.83.6.1641 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011028028
74 rdf:type schema:CreativeWork
75 https://doi.org/10.1093/nar/15.13.5105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027547740
76 rdf:type schema:CreativeWork
 




Preview window. Press ESC to close (or click here)


...