Method for high-throughtput AFLP-based polymorphism detection


Ontology type: sgo:Patent     


Patent Info

DATE

2011-09-07T00:00

AUTHORS

VAN EIJK, MICHAEL JOSEPHUS THERESIA , SORENSEN, ANKER PREBEN , VAN SCHRIEK, MARCO GERARDUS MARIA

ABSTRACT

The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers. More... »

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