Agrobacterium-Mediated Method For Producing Transformed Plant


Ontology type: sgo:Patent     


Patent Info

DATE

2011-06-22T00:00

AUTHORS

ISHIDA YUJI , HIEI YUKOH

ABSTRACT

Disclosed is a method of producing a transformed plant by using a novel Agrobacterium strain. A method of producing a transformed plant by using a novel Agrobacterium strain which comprises: (i) a co-culture step wherein a plant material inoculated with the Agrobacterium strain is cultured in a co-culture medium; and (ii) a step wherein, without conducting callus growth culture or after conducting callus growth culture, the tissue obtained in the above (i) is cultured in a redifferentiation medium to allow the plant to redifferentiate, characterized in that: 1) a treatment for enhancing transformation is conducted; and 2) in any step from the co-culture to the redifferentiation, no procedure of selecting transformed cells by taking advantage of the characteristic of the nucleic acid that has been transferred by the Agrobacterium strain is conducted. More... »

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