Agrobacterium-Mediated Method For Producing Transformed Plant


Ontology type: sgo:Patent     


Patent Info

DATE

2011-01-19T00:00

AUTHORS

ISHIDA, YUJI , HIEI, YUKOH

ABSTRACT

The present invention aims to provide a novel Agrobacterium-mediated method for producing a transformed plant.The method of the present invention is an Agrobacterium-mediated method for producing a transformed plant, which comprises: (i) a coculture step for culturing an Agrobacterium-inoculated plant material with a coculture medium; and (ii) a regeneration step for culturing the tissue obtained in (i) with a regeneration medium, either without callus growth culture or after callus growth culture, to thereby regenerate a whole plant, wherein 1) said method comprises transformation enhancement, and wherein 2) said method does not comprise selection of transformed cells based on the properties of a nucleic acid to be introduced by Agrobacterium in any step from coculture to regeneration. More... »

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The present invention aims to provide a novel Agrobacterium-mediated method for producing a transformed plant.

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\nwherein 1) said method comprises transformation enhancement, and
\nwherein 2) said method does not comprise selection of transformed cells based on the properties of a nucleic acid to be introduced by Agrobacterium in any step from coculture to regeneration.

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