Dna Array For Analyzing Dna Methylation, Method Of Constructing The Same And Mehtod Of Analyzing Dna Methylaion


Ontology type: sgo:Patent     


Patent Info

DATE

2008-05-14T00:00

AUTHORS

YAMAKAWA NAOMI

ABSTRACT

A method of constructing a DNA array for analyzing DNA modification (for example, methylation) comprising: (1) the step of preparing a DNA fragment mixture in which a modified base (for example, methylated cytosine) or a base (for example, cytosine) is exposed; (2) the step of contacting the DNA fragment mixture with an antibody against a modified base (for example, methylated cytosine) or an antibody against a base (for example, cytosine) and separating the mixture into a group consisting of immunocomplex-forming DNA fragments and another group consisting of unreacted DNA fragments, or a group consisting of DNA fragments showing high affinity for the antibody and another group consisting of DNA fragments showing low affinity for the antibody; (3) the step of identifying all or part of DNA fragments involved in each of the DNA fragment groups as described above; and (4) the step of providing nucleic acids hybridizable respectively with the thus identified DNA fragments on a substrate. More... »

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