Procaryotic Carbonyl Hydrolases And Mutants Thereof, And Methods For Producing And Using Them


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

BOTT, RICHARD RAY , Estell, David Aaron c/o Genencor International , FERRARI, EUGENIO , HENNER, DENNIS JAMES , Wells, James Allen c/o Genentech, Inc.

ABSTRACT

DNA sequences are disclosed encoding B. amyloliquefaciens and B. subtilis subtilisins and B. subtilis neutral protease. Using DNA derived from these sequences as hybridisation probes, genes for these and other closely related enzymes can be cloned from procaryotic sources, identified by their carbonyl hydrolytic acitivity and expressed in a suitable recombinant host. Moreover, the DNA can be mutated to produce enzymes with altered characteristics which can be screened for to identify desired changes, and thereafter the modified enzymes obtained by expression of the muted genes in suitable recombinant hosts. More... »

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