DNA probes for assaying B-lactamase production in gram-negative bacteria


Ontology type: sgo:Patent     


Patent Info

DATE

1989-01-25T00:00

AUTHORS

LEVESQUE, ROGER

ABSTRACT

There is provided a method for assaying the presence of a gene coding for the synthesis of β-lac­tamase in a targetted microorganism. This method comprises depositing and fixing on an inert support a sample containing DNA fragments in substantially single-stranded form and suspected of coding for the synthesis of β-lactamase; contacting the fixed single-stranded genetic material with a labelled probe having a nucleotide sequence of at least about 12 bases at least substantially complementary of a structural gene coding for the synthesis β-lactamase, the contacting being under hybridizing conditions at a pre-determined stringency; and detecting duplex formation on the support by means of the chosen label. There is also provided DNA probes comprising respectively 12, 15, 17 and 45 bases, these probes containing the conserved canonical aminoacid active site sequences common to β-lactamase genes OXA-2 and TEM-1, and DNA probes containing the conserved canonical aminoacid active site sequences common to the β-lactamase genes of OXA-1, PSE-1, ROB-1, OXA-3, OXA-4, ASF-2, PSE-4, CARB-3, CARB-4 and AER-1. More... »

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