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Experiments designed to define the differences between the 21 oncogene of DNA isolated from human bladder cancer cells and its corresponding protooncogene. By a series of in vitro recombinations, the difference was initially isolated to a 350 kb segment of DNA; sequencing defined the difference as a change in the Gly12 codon causing the p21 protein of the oncogene to contain valine at a location where the p21 protein of the proto- oncogene contained glycine. Assays for detecting carcinogenesis based on such differences are also described. In one type of assay, a restriction enzyme specific for either the altered or non-altered DNA segment of the genes are employed to detect carcinogenesis. In another type of assay, serological reagents, such as antibody specific for either p21 protein expressed from the proto-oncogene or oncogene, or a common site therein, are described.
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    "name": "Method of producing an antibody capable of detecting the presence of a human oncogene.", 
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49 TRIPLES      14 PREDICATES      19 URIs      8 LITERALS      2 BLANK NODES