Method of detecting electrophoretically separated oligonucleotides


Ontology type: sgo:Patent     


Patent Info

DATE

1987-08-19T00:00

AUTHORS

FUNG, STEVEN , WOO, SAM LEE , MENCHEN, STEVEN M. , CONNELL, CHARLES R. , HEINER,CHERYL

ABSTRACT

A method is provided for detecting up to four classes of oligonucleotides which have been separated by gel electrophoresis. The method entails labeling members of each class of oligonucleotide with dyes selected from separate sets of dyes so that members of the same class are labeled with dyes from the same set. The four sets of dyes of the invention consist of derivatives of fluorescein, 2',7'-dimethoxy-4',5'--dichlorofluorescein, tetramethylrhodamine, and rhodamine X carboxylic or sulfonic acid, respectively. Dyes from these sets are spectrally resolvable under conditions of gel electrophoresis. More... »

Related SciGraph Publications

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2471", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "name": "FUNG, STEVEN", 
        "type": "Person"
      }, 
      {
        "name": "WOO, SAM LEE", 
        "type": "Person"
      }, 
      {
        "name": "MENCHEN, STEVEN M.", 
        "type": "Person"
      }, 
      {
        "name": "CONNELL, CHARLES R.", 
        "type": "Person"
      }, 
      {
        "name": "HEINER,CHERYL", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1093/nar/13.12.4485", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037525378"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/321674a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041827788", 
          "https://doi.org/10.1038/321674a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/13.7.2399", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043931432"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1987-08-19T00:00", 
    "description": "

A method is provided for detecting up to four classes of oligonucleotides which have been separated by gel electrophoresis. The method entails labeling members of each class of oligonucleotide with dyes selected from separate sets of dyes so that members of the same class are labeled with dyes from the same set. The four sets of dyes of the invention consist of derivatives of fluorescein, 2',7'-dimethoxy-4',5'--dichlorofluorescein, tetramethylrhodamine, and rhodamine X carboxylic or sulfonic acid, respectively. Dyes from these sets are spectrally resolvable under conditions of gel electrophoresis.

", "id": "sg:patent.EP-0233053-A2", "keywords": [ "method", "oligonucleotides", "class", "gel electrophoresis", "labeling", "dye", "separate set", "same class", "same set", "invention", "derivative", "fluorescein", "rhodamine", "acid", "condition" ], "name": "Method of detecting electrophoretically separated oligonucleotides", "recipient": [ { "id": "https://www.grid.ac/institutes/grid.418190.5", "type": "Organization" } ], "sameAs": [ "https://app.dimensions.ai/details/patent/EP-0233053-A2" ], "sdDataset": "patents", "sdDatePublished": "2019-04-18T10:23", "sdLicense": "https://scigraph.springernature.com/explorer/license/", "sdPublisher": { "name": "Springer Nature - SN SciGraph project", "type": "Organization" }, "sdSource": "s3://com-uberresearch-data-patents-target-20190320-rc/data/sn-export/402f166718b70575fb5d4ffe01f064d1/0000100128-0000352499/json_export_02043.jsonl", "type": "Patent" } ]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/patent.EP-0233053-A2'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/patent.EP-0233053-A2'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/patent.EP-0233053-A2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/patent.EP-0233053-A2'


 

This table displays all metadata directly associated to this object as RDF triples.

63 TRIPLES      15 PREDICATES      32 URIs      23 LITERALS      2 BLANK NODES

Subject Predicate Object
1 sg:patent.EP-0233053-A2 schema:about anzsrc-for:2471
2 schema:author Nf44ecc4cfd054930a67a6eb0e7c91f44
3 schema:citation sg:pub.10.1038/321674a0
4 https://doi.org/10.1093/nar/13.12.4485
5 https://doi.org/10.1093/nar/13.7.2399
6 schema:datePublished 1987-08-19T00:00
7 schema:description <p num="pa01">A method is provided for detecting up to four classes of oligonucleotides which have been separated by gel electrophoresis. The method entails labeling members of each class of oligonucleotide with dyes selected from separate sets of dyes so that members of the same class are labeled with dyes from the same set. The four sets of dyes of the invention consist of derivatives of fluorescein, 2',7'-dimethoxy-4',5'--dichlorofluorescein, tetramethylrhodamine, and rhodamine X carboxylic or sulfonic acid, respectively. Dyes from these sets are spectrally resolvable under conditions of gel electrophoresis.</p>
8 schema:keywords acid
9 class
10 condition
11 derivative
12 dye
13 fluorescein
14 gel electrophoresis
15 invention
16 labeling
17 method
18 oligonucleotides
19 rhodamine
20 same class
21 same set
22 separate set
23 schema:name Method of detecting electrophoretically separated oligonucleotides
24 schema:recipient https://www.grid.ac/institutes/grid.418190.5
25 schema:sameAs https://app.dimensions.ai/details/patent/EP-0233053-A2
26 schema:sdDatePublished 2019-04-18T10:23
27 schema:sdLicense https://scigraph.springernature.com/explorer/license/
28 schema:sdPublisher Nd1907189ac224456827946569f73892b
29 sgo:license sg:explorer/license/
30 sgo:sdDataset patents
31 rdf:type sgo:Patent
32 N0710e2986a8b485cb25122ab112490b3 rdf:first N9a024d6fad0d4fea82dcadb7037a1dd9
33 rdf:rest N1d90d3df86ec49d89cf9358b4b6db2c6
34 N1d90d3df86ec49d89cf9358b4b6db2c6 rdf:first N33299b8eb397407ebb838f232c018258
35 rdf:rest rdf:nil
36 N33299b8eb397407ebb838f232c018258 schema:name HEINER,CHERYL
37 rdf:type schema:Person
38 N680263f002b3411595bac8019cbb21c7 schema:name MENCHEN, STEVEN M.
39 rdf:type schema:Person
40 N985f636d065a485f8355ba80d26db800 rdf:first Nb818ad48b6754bf5add596f22de26feb
41 rdf:rest Na0f7b09b57b645f397ae64a4c9687a9b
42 N9a024d6fad0d4fea82dcadb7037a1dd9 schema:name CONNELL, CHARLES R.
43 rdf:type schema:Person
44 Na0f7b09b57b645f397ae64a4c9687a9b rdf:first N680263f002b3411595bac8019cbb21c7
45 rdf:rest N0710e2986a8b485cb25122ab112490b3
46 Nb818ad48b6754bf5add596f22de26feb schema:name WOO, SAM LEE
47 rdf:type schema:Person
48 Nd1907189ac224456827946569f73892b schema:name Springer Nature - SN SciGraph project
49 rdf:type schema:Organization
50 Nf44ecc4cfd054930a67a6eb0e7c91f44 rdf:first Nf68bdac74f714c0f8c320d599937f95c
51 rdf:rest N985f636d065a485f8355ba80d26db800
52 Nf68bdac74f714c0f8c320d599937f95c schema:name FUNG, STEVEN
53 rdf:type schema:Person
54 anzsrc-for:2471 schema:inDefinedTermSet anzsrc-for:
55 rdf:type schema:DefinedTerm
56 sg:pub.10.1038/321674a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041827788
57 https://doi.org/10.1038/321674a0
58 rdf:type schema:CreativeWork
59 https://doi.org/10.1093/nar/13.12.4485 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037525378
60 rdf:type schema:CreativeWork
61 https://doi.org/10.1093/nar/13.7.2399 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043931432
62 rdf:type schema:CreativeWork
63 https://www.grid.ac/institutes/grid.418190.5 schema:Organization
 




Preview window. Press ESC to close (or click here)


...