Cloning Vehicle


Ontology type: sgo:Patent     


Patent Info

DATE

1987-08-19T00:00

AUTHORS

BACKMAN, KEITH C.

ABSTRACT

DNA capabable of transforming a cell to allow regulated in vivo formation of a protein-producing DNA segment com­ prising at least two subsegments positioned so that a specified end of one subsegment is adjacent a specified end of the other subsegment: the clonable DNA comprises: a first DNA sequence comprising one subsegment, the speci­ fied end of which is adjacent a DNA site for the enzymatic site-specific recombination; a second DNA sequence com­ prising the other subsegment, the specified end of which is adjacent a second DNA site for enzymatic site-specific recombination, said second enzyme-specific site being re­ mote from said first enzyme-specific site, whereby in vivo exposure of a population of the transforming DNA to a selected enzyme system triggers synchronized DNA rear­ rangement to form the protein-producing DNA segment, and to favour such formation over enzymatic reversal of the DNA rearrangement to reform the subsegments. Methods of using the DNA and precursors to the DNA are also disclosed. Cells used in the transformation are also disclosed. More... »

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