T4 Dna Fragment As A Stabilizer For Proteins Expressed By Cloned Dna


Ontology type: sgo:Patent     


Patent Info

DATE

N/A

AUTHORS

SIMON, LEE D. , FAY, ROSE B.

ABSTRACT

T4 DNA FRAGMENT AS A STABILIZER FOR PROTEINS EXPRESSED BY CLONED DNA Abstract of the Disclosure A DNA fragment isolated from the DNA of T-even bacteri-ophage which suppresses the degradation of abnormalproteins in bacterial cells is disclosed. The DNAfragment can be isolated by treating the DNA of T-evenbacteriophage with a restriction enzyme to cleave the DNA into fragments, at least one of said fragments consist-ing essentially of a sequence of nucleotide bases whichcode for protein that functions to suppress degradationof abnormal proteins in bacterial cells, inserting the DNA fragments into cloning vehicles, such as plasmids,to produce hybrid cloning vehicles, transforming bac-terial cells by introducing the hybrid cloning vehiclestherein, and selecting the transformants which exhibitsuppressed protein degradation mechanisms. The DNAfragment can then be isolated from the selected trans-formants. The stabilizing DNA fragment can be intro-duced into bacterial host cells to increase the yieldof foreign proteins expressed by cloned DNA. More... »

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T4 DNA FRAGMENT AS A STABILIZER FOR
PROTEINS EXPRESSED BY CLONED DNA

Abstract of the Disclosure

A DNA fragment isolated from the DNA of T-even bacteri-
ophage which suppresses the degradation of abnormal
proteins in bacterial cells is disclosed. The DNA
fragment can be isolated by treating the DNA of T-even
bacteriophage with a restriction enzyme to cleave the
DNA into fragments, at least one of said fragments consist-
ing essentially of a sequence of nucleotide bases which
code for protein that functions to suppress degradation
of abnormal proteins in bacterial cells, inserting the
DNA fragments into cloning vehicles, such as plasmids,
to produce hybrid cloning vehicles, transforming bac-
terial cells by introducing the hybrid cloning vehicles
therein, and selecting the transformants which exhibit
suppressed protein degradation mechanisms. The DNA
fragment can then be isolated from the selected trans-
formants. The stabilizing DNA fragment can be intro-
duced into bacterial host cells to increase the yield
of foreign proteins expressed by cloned DNA.


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