Process for producing an insulin precursor


Ontology type: sgo:Patent     


Patent Info

DATE

1981-10-07T00:00

AUTHORS

FRANK, BRUCE HILL

ABSTRACT

A process for preparing an insulin precursor is described, of the formula in which R is hydrogen, a chemically or enzymatically cleavable amino acid residue, or a chemically or enzymatically cleavable peptide moiety having at least two amino acid residues; Y is in which Z is Ala, Thr, or Ser; the moiety from A-1 to A-21 is an insulin A-chain; the moiety from B-1 to B-30 is an insulin B-chain; and X is a moiety which is joined to the insulin A-chain at the amino group of A-1 and to the insulin B-chain at the ε-amino group of B-29 or the carboxyl group of B-30, which moiety can be enzymatically or chemically cleaved from and without disruption of both the A-chain and the B-chain, which comprises reacting an S-sulfonate of the formula in which R, X, and Y are as defined above, with a mercaptan in an amount which provides from about 1 to about 5 -SH moieties per each -SSO3- moiety in an aqueous medium at a pH of from about 7 to about 11.5 and at an S-sulfonate concentration of up to about 10 mg. per ml. of aqueous medium. More... »

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