Isolation, separation and purification


Ontology type: npg:Subject  | skos:Concept     


Concept Info

NAME

Isolation, separation and purification

DESCRIPTION

Isolation, separation and purification refer to techniques used to isolate, concentrate or purify cells, viruses, cell fractions, organelles or biological macromolecules (e.g. proteins, protein complexes, chromatin, nucleic acids, carbohydrates or lipids) for subsequent analysis.

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70 sg:ontologies/subjects/sequencing
71 sg:ontologies/subjects/software
72 sg:ontologies/subjects/spectroscopy
73 sg:ontologies/subjects/structure-determination
74 skos:prefLabel Biological techniques
75 sg:ontologies/subjects/blotting sgo:sdDataset onto_subjects
76 rdf:type npg:Subject
77 skos:Concept
78 rdfs:label Blotting
79 skos:broader sg:ontologies/subjects/isolation-separation-purification
80 skos:definition Blotting is a technique for detecting nucleic acids or proteins by transferring them to a filter membrane. Molecules may be added directly to the membrane or separated by gel electrophoresis before transfer. Blotted nucleic acids are identified using labelled probes, whereas specific proteins are identified by immunodetection.
81 skos:inScheme sg:ontologies/subjects/
82 skos:prefLabel Blotting
83 sg:ontologies/subjects/chromatography sgo:sdDataset onto_subjects
84 rdf:type npg:Subject
85 skos:Concept
86 rdfs:label Chromatography
87 skos:altLabel Chromatographies
88 skos:broader sg:ontologies/subjects/isolation-separation-purification
89 skos:definition Chromatography is a technique used to separate mixtures of chemical compounds or biomolecules based on differences in their relative affinities for mobile (gas or liquid) and stationary (solid or liquid) phases. Chromatography is used for both preparative and analytical purposes and can be performed in column or planar format.
90 skos:inScheme sg:ontologies/subjects/
91 skos:prefLabel Chromatography
92 sg:ontologies/subjects/electrophoresis sgo:sdDataset onto_subjects
93 rdf:type npg:Subject
94 skos:Concept
95 rdfs:label Electrophoresis
96 skos:altLabel Electrophoreses
97 Gel electrophoresis
98 skos:broader sg:ontologies/subjects/isolation-separation-purification
99 skos:definition Electrophoresis is the process by which large charged molecules travel through a medium under a uniform electric field. The most common implementation, gel electrophoresis, is a method used to separate nucleic acids by length or proteins by size, conformation and charge based on their migration through a porous matrix.
100 skos:inScheme sg:ontologies/subjects/
101 skos:prefLabel Electrophoresis
102 sg:ontologies/subjects/elisa sgo:sdDataset onto_subjects
103 rdf:type npg:Subject
104 skos:Concept
105 rdfs:label ELISA
106 skos:altLabel Enzyme Linked Immunosorbent Assay
107 Enzyme-Linked Immunosorbent Assay
108 Enzyme-Linked Immunosorbent Assays
109 skos:broader sg:ontologies/subjects/immunological-techniques
110 sg:ontologies/subjects/isolation-separation-purification
111 skos:definition The enzyme-linked immunosorbent assay (ELISA) is a sensitive method to quantify or test for the presence of antigen or ligand. Enzyme-linked antibodies (or ligand binding proteins) are used to bind an analyte on a solid substrate, and convert a colourless reporter molecule to a coloured or luminescent product for detection.
112 skos:inScheme sg:ontologies/subjects/
113 skos:prefLabel ELISA
114 sg:ontologies/subjects/protein-enrichment sgo:sdDataset onto_subjects
115 rdf:type npg:Subject
116 skos:Concept
117 rdfs:label Protein enrichment
118 skos:broader sg:ontologies/subjects/isolation-separation-purification
119 skos:definition Protein enrichment is a technique by which proteins of a particular type in a biological sample are concentrated for further analysis and identification. Enrichment is used to help concentrate low-abundance proteins to improve their downstream analysis.
120 skos:inScheme sg:ontologies/subjects/
121 skos:prefLabel Protein enrichment
122 sg:ontologies/subjects/protein-purification sgo:sdDataset onto_subjects
123 rdf:type npg:Subject
124 skos:Concept
125 rdfs:label Protein purification
126 skos:altLabel Native Protein Purification
127 Protein Isolation
128 Recombinant Protein Purification
129 skos:broader sg:ontologies/subjects/isolation-separation-purification
130 skos:definition Protein purification is a technique by which a single protein type is isolated from a complex mixture such as a cell lysate. It can refer to purification of a native protein from a biological sample or of a recombinant protein using one of a variety of chemical, physical or biological methods.
131 skos:inScheme sg:ontologies/subjects/
132 skos:prefLabel Protein purification
133 sg:ontologies/subjects/western-blot sgo:sdDataset onto_subjects
134 rdf:type npg:Subject
135 skos:Concept
136 rdfs:label Western blot
137 skos:altLabel Western
138 Western Blots
139 Western Blotting
140 Western Blottings
141 Western Immunoblot
142 Western Immunoblots
143 Western Immunoblotting
144 Western Immunoblottings
145 skos:broader sg:ontologies/subjects/isolation-separation-purification
146 skos:definition The western blot is a method for detecting the presence and size of specific proteins. Proteins are first separated according to size or other physical properties by gel electrophoresis, then transferred to a membrane (blotted) before a detection step that uses labelled antibody to recognize the protein of interest.
147 skos:inScheme sg:ontologies/subjects/
148 skos:prefLabel Western blot
149 skos:Concept sgo:sdDataset for_codes
150 rdf:type rdfs:Class
151 rdfs:Resource
152 rdfs:subClassOf rdfs:Resource
153 skos:Concept
 




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