Immunological techniques


Ontology type: npg:Subject  | skos:Concept     


Concept Info

NAME

Immunological techniques

DESCRIPTION

Immunological techniques include both experimental methods to study the immune system and methods to generate or use immunological reagents as experimental tools. The most common immunological methods relate to the production and use of antibodies to detect specific proteins in biological samples.

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This table displays all metadata directly associated to this object as RDF triples.

192 TRIPLES      10 PREDICATES      27 URIs      13 LITERALS

Subject Predicate Object
1 sg:ontologies/subjects/immunological-techniques sgo:license sg:explorer/license/
2 sgo:sdDataset onto_subjects
3 rdf:type npg:Subject
4 skos:Concept
5 rdfs:label Immunological techniques
6 skos:altLabel Antibody Dissociation
7 Antibody Dissociations
8 Immunologic Technic
9 Immunologic Technics
10 Immunologic Technique
11 Immunologic Techniques
12 Immunological Technic
13 Immunological Technics
14 Immunological Technique
15 skos:broader sg:ontologies/subjects/biological-techniques
16 skos:definition Immunological techniques include both experimental methods to study the immune system and methods to generate or use immunological reagents as experimental tools. The most common immunological methods relate to the production and use of antibodies to detect specific proteins in biological samples.
17 skos:inScheme sg:ontologies/subjects/
18 skos:narrower sg:ontologies/subjects/antibody-generation
19 sg:ontologies/subjects/antibody-isolation-and-purification
20 sg:ontologies/subjects/elisa
21 sg:ontologies/subjects/elispot
22 sg:ontologies/subjects/immunoblotting
23 sg:ontologies/subjects/immunohistochemistry
24 sg:ontologies/subjects/immunoprecipitation
25 sg:ontologies/subjects/isolation-of-immune-cells
26 skos:prefLabel Immunological techniques
27 sg:ontologies/subjects/ dcterms:description The Nature Subjects Taxonomy is a polyhierarchical categorization of scholarly subject areas which are used for the indexing of content by Springer Nature.
28 dcterms:title Nature Subjects Taxonomy
29 sgo:sdDataset onto_subjects
30 rdf:type skos:ConceptScheme
31 skos:hasTopConcept sg:ontologies/subjects/DEPRECATED
32 sg:ontologies/subjects/biological-sciences
33 sg:ontologies/subjects/business-and-commerce
34 sg:ontologies/subjects/earth-and-environmental-sciences
35 sg:ontologies/subjects/health-sciences
36 sg:ontologies/subjects/humanities
37 sg:ontologies/subjects/physical-sciences
38 sg:ontologies/subjects/scientific-community-and-society
39 sg:ontologies/subjects/social-science
40 sg:ontologies/subjects/antibody-generation sgo:sdDataset onto_subjects
41 rdf:type npg:Subject
42 skos:Concept
43 rdfs:label Antibody generation
44 skos:altLabel Antibody production
45 skos:broader sg:ontologies/subjects/immunological-techniques
46 skos:definition Antibody generation is the production of antibodies from cells or animals in sufficient quantity to be used as an experimental or therapeutic reagent. Polyclonal antibody mixtures are generated by injecting an animal with an agent that elicits an immune response, and monoclonal antibodies are generated from immortal immune cell lines.
47 skos:inScheme sg:ontologies/subjects/
48 skos:prefLabel Antibody generation
49 sg:ontologies/subjects/antibody-isolation-and-purification sgo:sdDataset onto_subjects
50 rdf:type npg:Subject
51 skos:Concept
52 rdfs:label Antibody isolation and purification
53 skos:broader sg:ontologies/subjects/immunological-techniques
54 skos:definition Antibody isolation and purification is the concentration of an antibody or antibodies that recognize a specific target molecule and the removal of non-specific antibodies and contaminants. Pure isolated antibodies tend to have greater activity and are less likely to cross-react with non-target molecules than impure mixtures.
55 skos:inScheme sg:ontologies/subjects/
56 skos:prefLabel Antibody isolation and purification
57 sg:ontologies/subjects/biological-techniques sgo:sdDataset onto_subjects
58 rdf:type npg:Subject
59 skos:Concept
60 rdfs:label Biological techniques
61 skos:altLabel Methodological Studies
62 Methodological Study
63 Methods
64 Procedure
65 Procedures
66 skos:broader sg:ontologies/subjects/biological-sciences
67 skos:definition Biological techniques are methods or procedures that are used to study living things. They include experimental and computational methods, approaches, protocols and tools for biological research.
68 skos:inScheme sg:ontologies/subjects/
69 skos:narrower sg:ontologies/subjects/analytical-biochemistry
70 sg:ontologies/subjects/behavioural-methods
71 sg:ontologies/subjects/bioinformatics
72 sg:ontologies/subjects/biological-models
73 sg:ontologies/subjects/biophysical-methods
74 sg:ontologies/subjects/cytological-techniques
75 sg:ontologies/subjects/electrophysiology
76 sg:ontologies/subjects/epigenetics-analysis
77 sg:ontologies/subjects/experimental-organisms
78 sg:ontologies/subjects/gene-delivery
79 sg:ontologies/subjects/gene-expression-analysis
80 sg:ontologies/subjects/genetic-engineering
81 sg:ontologies/subjects/genetic-techniques
82 sg:ontologies/subjects/genomic-analysis
83 sg:ontologies/subjects/high-throughput-screening
84 sg:ontologies/subjects/imaging
85 sg:ontologies/subjects/immunological-techniques
86 sg:ontologies/subjects/isolation-separation-purification
87 sg:ontologies/subjects/lab-on-a-chip
88 sg:ontologies/subjects/mass-spectrometry
89 sg:ontologies/subjects/metabolomics
90 sg:ontologies/subjects/microbiology-techniques
91 sg:ontologies/subjects/microscopy
92 sg:ontologies/subjects/molecular-engineering
93 sg:ontologies/subjects/nanobiotechnology
94 sg:ontologies/subjects/optogenetics
95 sg:ontologies/subjects/proteomic-analysis
96 sg:ontologies/subjects/sensors-and-probes
97 sg:ontologies/subjects/sequencing
98 sg:ontologies/subjects/software
99 sg:ontologies/subjects/spectroscopy
100 sg:ontologies/subjects/structure-determination
101 skos:prefLabel Biological techniques
102 sg:ontologies/subjects/elisa sgo:sdDataset onto_subjects
103 rdf:type npg:Subject
104 skos:Concept
105 rdfs:label ELISA
106 skos:altLabel Enzyme Linked Immunosorbent Assay
107 Enzyme-Linked Immunosorbent Assay
108 Enzyme-Linked Immunosorbent Assays
109 skos:broader sg:ontologies/subjects/immunological-techniques
110 sg:ontologies/subjects/isolation-separation-purification
111 skos:definition The enzyme-linked immunosorbent assay (ELISA) is a sensitive method to quantify or test for the presence of antigen or ligand. Enzyme-linked antibodies (or ligand binding proteins) are used to bind an analyte on a solid substrate, and convert a colourless reporter molecule to a coloured or luminescent product for detection.
112 skos:inScheme sg:ontologies/subjects/
113 skos:prefLabel ELISA
114 sg:ontologies/subjects/elispot sgo:sdDataset onto_subjects
115 rdf:type npg:Subject
116 skos:Concept
117 rdfs:label ELISPOT
118 skos:altLabel Enzyme Linked Immunospot Assay
119 Enzyme-Linked Immunospot Assay
120 Enzyme-Linked Immunospot Assays
121 skos:broader sg:ontologies/subjects/immunological-techniques
122 skos:definition The enzyme-linked immunosorbent spot (ELISPOT) assay is a sensitive method to count cells of a specific type by capturing molecules that they secrete on an immunosorbent surface and detecting them with the ELISA method. It is commonly used to count immune cells based on the secretion of cytokines.
123 skos:inScheme sg:ontologies/subjects/
124 skos:prefLabel ELISPOT
125 sg:ontologies/subjects/immunoblotting sgo:sdDataset onto_subjects
126 rdf:type npg:Subject
127 skos:Concept
128 rdfs:label Immunoblotting
129 skos:altLabel Dot Immunoblotting
130 Dot Immunoblottings
131 Electroimmunoblotting
132 Electroimmunoblottings
133 Immunoblottings
134 Immunoelectroblotting
135 Immunoelectroblottings
136 Reverse Immunoblotting
137 Reverse Immunoblottings
138 skos:broader sg:ontologies/subjects/immunological-techniques
139 skos:definition Immunoblotting is an in vitro technique for detecting specific proteins using antibodies. Protein mixtures are transferred to a filter membrane either directly or after separation by electrophoresis, then probed with antibodies that are either labelled (e.g. with fluorophore or an enzyme) or bound by a labelled secondary antibody for detection.
140 skos:inScheme sg:ontologies/subjects/
141 skos:prefLabel Immunoblotting
142 sg:ontologies/subjects/immunohistochemistry sgo:sdDataset onto_subjects
143 rdf:type npg:Subject
144 skos:Concept
145 rdfs:label Immunohistochemistry
146 skos:altLabel Immunocytochemistry
147 Immunogold Silver Technics
148 Immunogold Silver Techniques
149 Immunogold Technic
150 Immunogold Technics
151 Immunogold Technique
152 Immunogold Techniques
153 Immunogold-Silver Technic
154 Immunogold-Silver Technics
155 Immunogold-Silver Technique
156 Immunogold-Silver Techniques
157 Immunohistocytochemistry
158 Immunolabeling Technic
159 Immunolabeling Technics
160 Immunolabeling Technique
161 Immunolabeling Techniques
162 skos:broader sg:ontologies/subjects/histocytochemistry
163 sg:ontologies/subjects/immunological-techniques
164 skos:definition Immunohistochemistry is a technique for detecting molecules of interest within tissues using antibodies. Probes or enzymes conjugated to primary antibodies that bind the target molecule (or secondary antibodies that recognize the primary antibody) report the location of targets in tissue or cell samples for imaging.
165 skos:inScheme sg:ontologies/subjects/
166 skos:prefLabel Immunohistochemistry
167 sg:ontologies/subjects/immunoprecipitation sgo:sdDataset onto_subjects
168 rdf:type npg:Subject
169 skos:Concept
170 rdfs:label Immunoprecipitation
171 skos:altLabel Co Immunoprecipitation
172 Co-Immunoprecipitation
173 Co-Immunoprecipitations
174 Immune Precipitation
175 Immune Precipitations
176 skos:broader sg:ontologies/subjects/immunological-techniques
177 skos:definition Immunoprecipitation is a method for isolating a target molecule from a solution or cell lysate using an antibody that recognizes it. In the most common version, binding of antibody to a peptide antigen forms an aggregate (including any binding partners or complexes) that precipitates and can be concentrated by centrifugation.
178 skos:inScheme sg:ontologies/subjects/
179 skos:prefLabel Immunoprecipitation
180 sg:ontologies/subjects/isolation-of-immune-cells sgo:sdDataset onto_subjects
181 rdf:type npg:Subject
182 skos:Concept
183 rdfs:label Immune cell isolation
184 skos:broader sg:ontologies/subjects/immunological-techniques
185 skos:definition Immune cell isolation refers to methods that enable the enrichment of immune cell subsets using antibody-mediated recognition of specific cell surface antigens, followed by sorting or separation with techniques such as flow cytometry, density centrifugation or magnetic isolation.
186 skos:inScheme sg:ontologies/subjects/
187 skos:prefLabel Immune cell isolation
188 skos:Concept sgo:sdDataset for_codes
189 rdf:type rdfs:Class
190 rdfs:Resource
191 rdfs:subClassOf rdfs:Resource
192 skos:Concept
 




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