Regulation Mechanism of CD147 Glycosylation Modification and Its Function in Melanoma Invasion and Metastasis


Ontology type: schema:MonetaryGrant     


Grant Info

YEARS

2017-2021

FUNDING AMOUNT

2400000.0 CNY

ABSTRACT

Tumor microenvironment plays critical role in tumorigenesis,which is controlled by metabolism. The glycosylation of molecules in membrane and cellular glycolysis orchestrate formation of tumor microenvironment. Our preliminary data showed CD147 majorly distributed in cytoplasm in metastatic melanoma tissue comparing to membrane distribution in primary melanoma. We proposed glycosylation alteration of CD147 plays a key role in this phenomena. It was reported that CD147 effects on cell adhesion, pH value of microenvironment, cytokine secretion which affects on tumor microenvironment. We have plan to identify the CD147 interaction proteins in cytoplasm through yeast two hybrid and IP-MS and study the cytoplasmic CD147 function. The glycosylation sites of CD147 in clinical metastatic melanoma tissue would be identified by IP-MS. Mutation of CD147 at glycosylation sites would be generated and be constructed into melanoma animal model to investigate the glycosylation function of CD147 in melanoma. Combination of primary cultured melanoma cell lines and PDX model will be performed to confirm results from melanoma cell lines. The purpose of this application is how glycosylation affects on CD147 cytoplasmic distribution and function, which promotes melanoma progression through tumor microenvironment. More... »

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