Study on Recognition Mechanism of Amidase Substrate Based on Molecular Transformation View Homepage


Ontology type: schema:MonetaryGrant     


Grant Info

YEARS

2017-2019

FUNDING AMOUNT

200000.0 CNY

ABSTRACT

Amidase plays an important role in the synthesis of chiral carboxylic acids and amide derivatives, which makes it one of the key enzymes in organic chemical production. In this project, an amidase from Rhodococcus erythropolis ZJB-09149, which has important prospect for industrial applications, will be used for fundamental studies, alongside halogen-substituted nicotinamides and halogen-substituted mandelamines as substrates. According to the steric sensitivity of amidase, molecular modification approach will be adopted to improve the amidase’s catalytic activity and stereoselectivity. The study of changes in catalytic activity and stereoselectivity caused by different mutation sites on amidase, could reveal the relationship between the enzyme structure and its effect on the catalytic activity and stereoselectivity. By that, it could pinpoint the amino acids participated in the catalysis, and further understanding of its structure-function relationship and substrate recognition mechanism. This subject aims to provide more insights on the catalytic mechanism of amidase and broaden its potential in terms of application. More... »

URL

http://output.nsfc.gov.cn/conclusionProject/9e2c1a5acc1c8cc9f3d530953eda655a

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