Estrogen-induced microenvironment enhances limbal stem cell stem cell apoptosis by regulating telomerase pathway and its mechanism of inhibiting apoptosis View Homepage


Ontology type: schema:MonetaryGrant     


Grant Info

YEARS

2013-2016

FUNDING AMOUNT

700000 CNY

ABSTRACT

The embryonic stem cell micro-environment plays an important role in postponing the somatic cells senescence and promoting their proliferation. Our previous work has demonstrated that the proliferative capacities of rabbit corneal epithelial cells and human corneal endothelial cells were promoted by embryonic stem cell-conditioned medium, based on this, combining that our findings that telomerase rejuvenated in the cytoplasm of corneal epithelial cells cultured in embryonic stem cell-conditioned medium, we propose to confirm and consummate our hypothesis that functional properties of stem-like corneal epithelial cells can be enhanced by co-culturing with embryonic stem cells via activation of the integrinβ1-FAK-PI3K/Akt signaling pathway from the three following aspects:1. to verify whether ES micro-environment maintains or regains stemness of human limbal stem cells and inhibits their apotosis via the maintenance of telomerase activity; 2. to confirm the roles of telomerase and FAK/Wnt signaling pathways in the maintenance of biological characteristics in human limbal stem cells; 3. to figure out the definite effects of telomerase-p21-mitochondrial axis in inhibiting apoptosis in human limbal stem cells. This project will focus on the scientific problem "the stemness maintenance in human limbal stem cells", aim More... »

URL

http://npd.nsfc.gov.cn/projectDetail.action?pid=81270971

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