Receptor Free Tagged FRET Based on NIR-QDs and Its Clinical Blood Sample Analysis View Homepage


Ontology type: schema:MonetaryGrant     


Grant Info

YEARS

2013-2016

FUNDING AMOUNT

250000 CNY

ABSTRACT

Fluorescence resonance energy transfer (FRET) is a highly sensitive and homogeneous assay technology. However, the assay sensitivity in blood sample will be lowered, which is mainly attributed to the autofluorescence,scattering light and light absorption arise from biological matrix. Additionally, the energy acceptors like organic dyes or gold nanoparticles in FRET usually need complicated and time-consuming biological label or surface modification. Aim at improving analytical performance of FRET assay, especially in complicated clinical blood sample, NIR-emitting Quantum dots (QDs) and nanoquencher with unique structure and electronic properties are adopted. The project is proposed to develop new FRET strategy with aptamer/peptide tagged NIR emitting QDs as the donor and inorganic nanoquencher (carbon nanoparticles, aromatic polymers) as the label-free acceptor. In the near-infrared (650 - 900 nm) detection window, the optical interference in biological sample can be completelly eliminated, and thus, the ratio of S/N(signal to noise) is enhanced. The FRET process is induced by the π?π stacking interaction between aptamer/peptide and π-electron riched nanoquencher, which dexterously avoids biological label or surface modification typically existed in the traditional energy acceptors, resulting in low-cost and s More... »

URL

http://npd.nsfc.gov.cn/projectDetail.action?pid=21305152

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