Study on Substrate Specificity and Enantioselectivity of Amidase Enzyme Based on Enzyme Activity Fingerprint Analysis View Homepage


Ontology type: schema:MonetaryGrant     


Grant Info

YEARS

2013-2015

FUNDING AMOUNT

250000.0 CNY

ABSTRACT

Amidases are promising synthesis tools for chiral carboxylic acids and amide derivatives. Due to the lack of deep understanding of substrate specificity and enantioselectivity law, the development, application and reconstruction of amidase are severely restricted. This project focused on the new perspective of activity fingerprint analysis, to reveal the substrate specificity and enantioselectivity laws of amidase. By establishing typical amidase library, structure library and varied substituent group library, together with enzyme activity fingerprint analysis system with high resolution, the fingerprint map of substrate specificity and enantioselectivity can be obtained. The homologous modeling and molecular docking technologies will be further applied to study the effects of key amino acid sites of amidase structure on the activity and enantioselectivity to different substrates, to accurately position the amino acid residues and functional domains that affect the amidases' substrate recognition, stereoselective recognition and the acyl intermediate state formation, which can finally reveal the molecular mechanisms of its substrate specificity and enantioselectivity. The whole research plays an important role in the field of amidases' rational selection, design and reconstruction, which is benefit to the development of enantioselective biocatalytic synthesis methods of amidase. More... »

URL

http://output.nsfc.gov.cn/conclusionProject/dbe111464321fe47159de424c3488eda

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