From DNA to genome: New development on human evolution study by next-generation sequencer View Homepage


Ontology type: schema:MonetaryGrant     


Grant Info

YEARS

2013-2016

FUNDING AMOUNT

14950000 JPY

ABSTRACT

Due to the difficulties in deep sequencing, high-throughput sequencing of ancient DNA has been limited to exceptionally well-preserved ancient materials. The primary factor is microbial attack popularly observed in the buried materials, and it causes drastic increase in relative ratio of microbial DNA in the extracted DNA. I developed a unified strategy in which emulsion PCR is coupled with target enrichment followed by next-generation sequencing. The method made it possible to obtain efficiently non-duplicated reads mapped to target sequences of interest, and this can achieve deep and reliable sequencing of ancient DNA from typical materials, even though poorly preserved. Then, I applied this method to ancient human remains from archaeological sites of various times, and succeeded in obtaining highly reliable and entire nucleotide sequences of mitochondrial genome. More... »

URL

https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25291104

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