The new deployment of the development of the ancient genome analysis of Pleistocene animals relict body and phylogeography research View Homepage


Ontology type: schema:MonetaryGrant     


Grant Info

YEARS

2007-2008

FUNDING AMOUNT

3500000 JPY

ABSTRACT

For the purpose to clarify the system geography and system evolution of Japan mammalian phase, the Society year also, attempt for Honshu brown bear excavated bone specimen of the Late Pleistocene and gene amplification of the partial sequence of the mitochondrial DNA of the improvement of the analytical techniques It was. Using DNA extracted carefully to avoid contamination of foreign DNA, has been experimented with improving the reaction conditions of the gene amplification was the same result for the sample that was not successfully last year. This fossil DNA of Pleistocene shows that it is extremely destructive. The future, in the analyzed the animal heritage Son Tay of excavated Pleistocene from Japan inside and outside, to continue the improvement of analysis technology. On the other hand, about the human bones excavated from the ruins of the Jomon period and Okhotsk era of Hokkaido around, so far successful was tried the gene amplification of nuclear genes on the chromosome (such as earwax gene), in the mitochondrial DNA analysis for Okhotsk human bones since the successful, the DNA remaining situation is to choose a good specimen, was examined single nucleotide polymorphisms of the nuclear genes, a high rate in the earwax gene were able to this gene amplification. As a result, it was enough to be able to calculate the allele frequency of earwax gene in the Okhotsk people still Jomon people of a population of Hokkaido. By molecular phylogenetic analysis of mitochondrial DNA, but Okhotsk people is becoming clear that became the intermediary of the continent and Hokkaido of gene flow, of nuclear genes in ancient DNA analysis, from the continent through the Okhotsk people to Hokkaido gene flow has been shown. In the case of a single nucleotide polymorphism analysis, or those from the resulting gene amplification product really is extracted ancient DNA, or, if due to the foreign DNA, must be determined. To solve it, it is necessary to verify the reproducibility of the analytical results, was also the additional experiments. More... »

URL

https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19657028

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