The role of non-pylori heliobacter in de-novo paediatric inflammatory bowel disease View Homepage


Ontology type: schema:MonetaryGrant     


Grant Info

YEARS

2009-2012

FUNDING AMOUNT

182235.0 GBP

ABSTRACT

Non-pylori Helicobacter has been identified as a potential inflammatory bowel disease (IBD) pathogen. Animal work has initiated IBD-like disease using non-pylori Helicobacter and demonstrated a post-infection reduction in commensal bacteria and IgG response to commensal organisms, suggesting these organisms alter the intestinal microbiota. Zhang et al reported 92% non-pylori Helicobacter prevalence in paediatric IBD and 25% in controls suggesting a role in human IBD. Work by our group using fluorescent in-situ hybridisation (FISH) in colonic tissue has demonstrated a difference in the prevalence of these organisms in adults with ulcerative colitis (UC) versus asymptomatic adult controls (79% vs. 23%, p<0.001). An identical paediatric study showed a prevalence of 93% in UC patients against 60% in controls. We have also uniquely shown a cloud of non-pylori Helicobacter in the colon of a de-novo child with IBD. We aim to examine the role of non-pylori Helicobacter at onset of IBD, to culture these organisms for further work and to examine colonic bacterial diversity in de-novo paediatric IBD. Colonic biopsies from nationally recruited paediatric subjects with treatment-naive de-novo IBD and controls with a normal colon will be screened by nested fluorescent PCR, Southern-blot and FISH to identify non-pylori Helicobacter positive individuals prior to culturing to isolate and further analyse the non-pylori Helicobacter. We will also assess the differences in bacterial diversity between Crohn's, UC and control groups by denaturing gradient gel electrophoresis and cloning/sequencing approaches. As a secondary aim, we will assess the effect of non-pylori Helicobacter on overall colonic microbial diversity. More... »

URL

https://europepmc.org/grantfinder/grantdetails?query=gid%3A"CAF/08/01"

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