Isolation and functional ammonia oxidation in soil microbial analysis View Homepage


Ontology type: schema:MonetaryGrant     


Grant Info

YEARS

2008-2010

FUNDING AMOUNT

200000 CNY

ABSTRACT

Ammonia-oxidizing bacteria (AOB)and Ammonia-oxidizing archaea (AOA) are the two main groups of ammonia-oxidizing microorganisms in soil. In this project, AOB and AOA from different soils were cultured to learn their physiological characters and functions. Several groups of AOB were accumulated and their physiological characters were preliminary understood. The culture of AOA from soil is still unsuccessful in the world. In our study, although lots of work were done to culture the AOA, growth of AOA was only detected in natural medium (sterile soil), and accumulation of AOA is very difficult. In order to better understand the important impact factors related to and the effects of human activities on the composition and function of AOA and AOB, abundance and community structure of AOB and AOA and ammonia oxidization rate were analyzed in several soils with different land utilization patterns, N addition rates or soil pH. The composition of AOB and AOA and ammonia oxidization rate can be affected by several human activities, while soil pH and ammonia concentration were shown to be the main impact factors. AOB abundance was mainly determined by ammonia concentration, and correlated with ammonia oxidization rate when there was no significant change in soil pH, while AOA abundance was not positively correlated with ei More... »

URL

http://npd.nsfc.gov.cn/projectDetail.action?pid=40701087

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