Study Chinese Valsa species of bacteria and pathogenicity difference View Homepage


Ontology type: schema:MonetaryGrant     


Grant Info

YEARS

2007-2010

FUNDING AMOUNT

300000 CNY

ABSTRACT

Valsa canker is one of the most destructive diseases of apple in eastern Asia,especially in China. Valsa mali was confirmed to be an independent species and a causal pathogen of Valsa canker on apple and pear in China by comparing with morphology and rDNA-ITS sequences. Significant intraspecific genetic differentiation was detected in V. mali with two varieties recognized, V. mali var. mali occurring exclusively on apple and V. mali var. pyri occurring on both apple and pear. Additionally, a nested PCR assay system was established, which achieved to specific, sensitive and rapid detection of latent Valsa canker pathogens. Furthermore, an sensitively indoor method for disease severity screening was developed by inoculating detached expanded leaves and young shoots at room temperature. The isolates pathogenicity collected from China were screened and the results showed the differentiation in pathogenicity on two species of Valsa spp.and on V. mali in China. The difference of pathogenicity was not found related to geographical location of the isolates. Our histological and cytological studies revealed the infection behavior and development process of pathogen in apple bark. Conidium swelled and developed 1-3 germ tube in 24h. The germinated conidia were observed to penetrate the host by various wound. After penetra More... »

URL

http://npd.nsfc.gov.cn/projectDetail.action?pid=30771396

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