LGR receptors mark adult stem cells in multiple mammalian tissues View Homepage


Ontology type: schema:MonetaryGrant     


Grant Info

YEARS

2009-2014

FUNDING AMOUNT

2106000 EUR

ABSTRACT

Self-renewal and repair of tissues in adult mammals is fueled by rare tissue stem cells. Identification and subsequent study of such adult stem cells depends strictly on the availability of molecular markers. To date, a paucity of single definitive markers has complicated the study of the biology of these rare cells. Our interest in the role of the Wnt pathway in intestinal homeostasis and cancer has led us to exhaustively define the Wnt target gene program shared between these two processes. Within this program, the cell surface receptor-encoding Lgr5 represented a candidate stem cell marker for the intestine. The creation of Lgr5-genetic mouse models subsequently allowed us to definitively identify the Lgr5+ cells as the intestinal stem cells. These stem cells divide every day, yet persist throughout life. Using the same mouse models, we have found that Lgr5 marks stem cells in a variety of other tissues. For two family members with similarly restricted expression domains, Lgr4 and -6, comparable genetic tools have been generated. In this proposal, I aim to exploit these unique genetic tools to study primary adult stem cells in health and disease. This should allow us to outline unique and common characteristics of individual stem cell types, and possibly- define the minimum molecular determinants of stemness. Specifically, I aim: 1. To make a complete inventory of adult stem cells expressing Lgr4, Lgr5 and/or Lgr6 using the transgenic mouse models. 2. To FACS-purify stem cells from selected tissues, and detail their molecular and metabolic characteristics. 3. To genetically define the biological role of the Lgr-family receptors in adult stem cells by gene knockout. 4. To translate these findings to the equivalent human tissues using antibody-based histology and FACS technology. 5. To explore the usefulness of the Lgr proteins as markers of cancer stem cells in human solid tumors. More... »

URL

http://cordis.europa.eu/project/rcn/90072_en.html

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2206", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2206", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "amount": {
      "currency": "EUR", 
      "type": "MonetaryAmount", 
      "value": "2106000"
    }, 
    "description": "Self-renewal and repair of tissues in adult mammals is fueled by rare tissue stem cells. Identification and subsequent study of such adult stem cells depends strictly on the availability of molecular markers. To date, a paucity of single definitive markers has complicated the study of the biology of these rare cells. Our interest in the role of the Wnt pathway in intestinal homeostasis and cancer has led us to exhaustively define the Wnt target gene program shared between these two processes. Within this program, the cell surface receptor-encoding Lgr5 represented a candidate stem cell marker for the intestine. The creation of Lgr5-genetic mouse models subsequently allowed us to definitively identify the Lgr5+ cells as the intestinal stem cells. These stem cells divide every day, yet persist throughout life. Using the same mouse models, we have found that Lgr5 marks stem cells in a variety of other tissues. For two family members with similarly restricted expression domains, Lgr4 and -6, comparable genetic tools have been generated. In this proposal, I aim to exploit these unique genetic tools to study primary adult stem cells in health and disease. This should allow us to outline unique and common characteristics of individual stem cell types, and  possibly- define the minimum molecular determinants of stemness. Specifically, I aim: 1. To make a complete inventory of adult stem cells expressing Lgr4, Lgr5 and/or Lgr6 using the transgenic mouse models. 2. To FACS-purify stem cells from selected tissues, and detail their molecular and metabolic characteristics. 3. To genetically define the biological role of the Lgr-family receptors in adult stem cells by gene knockout. 4. To translate these findings to the equivalent human tissues using antibody-based histology and FACS technology. 5. To explore the usefulness of the Lgr proteins as markers of cancer stem cells in human solid tumors.", 
    "endDate": "2014-02-28T00:00:00Z", 
    "funder": {
      "id": "https://www.grid.ac/institutes/grid.452896.4", 
      "type": "Organization"
    }, 
    "id": "sg:grant.3777594", 
    "identifier": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "3777594"
        ]
      }, 
      {
        "name": "cordis_id", 
        "type": "PropertyValue", 
        "value": [
          "90072"
        ]
      }
    ], 
    "inLanguage": [
      "en"
    ], 
    "keywords": [
      "health", 
      "subsequent studies", 
      "paucity", 
      "biological role", 
      "intestine", 
      "such adult stem cells", 
      "gene knockout", 
      "complete inventory", 
      "Lgr5+ cells", 
      "LGR6", 
      "adult mammals", 
      "Wnt pathway", 
      "variety", 
      "histology", 
      "unique genetic tool", 
      "transgenic mouse model", 
      "identification", 
      "FACS technology", 
      "biology", 
      "Wnt target gene program", 
      "LGR5", 
      "creation", 
      "other tissues", 
      "Lgr proteins", 
      "individual stem cell types", 
      "multiple mammalian tissues", 
      "cell surface receptor-encoding Lgr5", 
      "primary adult stem cells", 
      "adult stem cells", 
      "metabolic characteristics", 
      "cells", 
      "tissue", 
      "interest", 
      "stemness", 
      "self-renewal", 
      "same mouse model", 
      "candidate stem cell markers", 
      "life", 
      "antibodies", 
      "findings", 
      "disease", 
      "program", 
      "minimum molecular determinants", 
      "days", 
      "study", 
      "equivalent human tissue", 
      "Lgr-family receptors", 
      "repair", 
      "rare cells", 
      "intestinal stem cells", 
      "process", 
      "LGR4", 
      "FACS", 
      "cancer", 
      "expression domains", 
      "family members", 
      "date", 
      "human solid tumors", 
      "usefulness", 
      "stem cells", 
      "markers", 
      "cancer stem cells", 
      "intestinal homeostasis", 
      "proposal", 
      "mark", 
      "common characteristics", 
      "rare tissue stem cells", 
      "single definitive markers", 
      "comparable genetic tools", 
      "role", 
      "molecular markers", 
      "Lgr5-genetic mouse models", 
      "LGR receptors", 
      "availability"
    ], 
    "name": "LGR receptors mark adult stem cells in multiple mammalian tissues", 
    "recipient": [
      {
        "id": "https://www.grid.ac/institutes/grid.418101.d", 
        "type": "Organization"
      }, 
      {
        "affiliation": {
          "id": "https://www.grid.ac/institutes/grid.418101.d", 
          "name": "KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPEN - KNAW", 
          "type": "Organization"
        }, 
        "familyName": "Clevers", 
        "givenName": "Johannes Carolus", 
        "id": "sg:person.01244052500.89", 
        "type": "Person"
      }, 
      {
        "member": "sg:person.01244052500.89", 
        "roleName": "PI", 
        "type": "Role"
      }
    ], 
    "sameAs": [
      "https://app.dimensions.ai/details/grant/grant.3777594"
    ], 
    "sdDataset": "grants", 
    "sdDatePublished": "2019-03-07T11:22", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com.uberresearch.data.processor/core_data/20181219_192338/projects/base/cordis_projects_2.xml.gz", 
    "startDate": "2009-03-01T00:00:00Z", 
    "type": "MonetaryGrant", 
    "url": "http://cordis.europa.eu/project/rcn/90072_en.html"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/grant.3777594'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/grant.3777594'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/grant.3777594'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/grant.3777594'


 

This table displays all metadata directly associated to this object as RDF triples.

118 TRIPLES      19 PREDICATES      96 URIs      88 LITERALS      5 BLANK NODES

Subject Predicate Object
1 sg:grant.3777594 schema:about anzsrc-for:2206
2 schema:amount N5b06381b5ecd4ceb8f9c899ff893bd19
3 schema:description Self-renewal and repair of tissues in adult mammals is fueled by rare tissue stem cells. Identification and subsequent study of such adult stem cells depends strictly on the availability of molecular markers. To date, a paucity of single definitive markers has complicated the study of the biology of these rare cells. Our interest in the role of the Wnt pathway in intestinal homeostasis and cancer has led us to exhaustively define the Wnt target gene program shared between these two processes. Within this program, the cell surface receptor-encoding Lgr5 represented a candidate stem cell marker for the intestine. The creation of Lgr5-genetic mouse models subsequently allowed us to definitively identify the Lgr5+ cells as the intestinal stem cells. These stem cells divide every day, yet persist throughout life. Using the same mouse models, we have found that Lgr5 marks stem cells in a variety of other tissues. For two family members with similarly restricted expression domains, Lgr4 and -6, comparable genetic tools have been generated. In this proposal, I aim to exploit these unique genetic tools to study primary adult stem cells in health and disease. This should allow us to outline unique and common characteristics of individual stem cell types, and possibly- define the minimum molecular determinants of stemness. Specifically, I aim: 1. To make a complete inventory of adult stem cells expressing Lgr4, Lgr5 and/or Lgr6 using the transgenic mouse models. 2. To FACS-purify stem cells from selected tissues, and detail their molecular and metabolic characteristics. 3. To genetically define the biological role of the Lgr-family receptors in adult stem cells by gene knockout. 4. To translate these findings to the equivalent human tissues using antibody-based histology and FACS technology. 5. To explore the usefulness of the Lgr proteins as markers of cancer stem cells in human solid tumors.
4 schema:endDate 2014-02-28T00:00:00Z
5 schema:funder https://www.grid.ac/institutes/grid.452896.4
6 schema:identifier N07dac15a077d47aa9a94fafefe5fe0e0
7 N357c8a06878749fb9b579cac7bd640f9
8 schema:inLanguage en
9 schema:keywords FACS
10 FACS technology
11 LGR receptors
12 LGR4
13 LGR5
14 LGR6
15 Lgr proteins
16 Lgr-family receptors
17 Lgr5+ cells
18 Lgr5-genetic mouse models
19 Wnt pathway
20 Wnt target gene program
21 adult mammals
22 adult stem cells
23 antibodies
24 availability
25 biological role
26 biology
27 cancer
28 cancer stem cells
29 candidate stem cell markers
30 cell surface receptor-encoding Lgr5
31 cells
32 common characteristics
33 comparable genetic tools
34 complete inventory
35 creation
36 date
37 days
38 disease
39 equivalent human tissue
40 expression domains
41 family members
42 findings
43 gene knockout
44 health
45 histology
46 human solid tumors
47 identification
48 individual stem cell types
49 interest
50 intestinal homeostasis
51 intestinal stem cells
52 intestine
53 life
54 mark
55 markers
56 metabolic characteristics
57 minimum molecular determinants
58 molecular markers
59 multiple mammalian tissues
60 other tissues
61 paucity
62 primary adult stem cells
63 process
64 program
65 proposal
66 rare cells
67 rare tissue stem cells
68 repair
69 role
70 same mouse model
71 self-renewal
72 single definitive markers
73 stem cells
74 stemness
75 study
76 subsequent studies
77 such adult stem cells
78 tissue
79 transgenic mouse model
80 unique genetic tool
81 usefulness
82 variety
83 schema:name LGR receptors mark adult stem cells in multiple mammalian tissues
84 schema:recipient N038c3bb8d04f49b2a219b68cc7807095
85 sg:person.01244052500.89
86 https://www.grid.ac/institutes/grid.418101.d
87 schema:sameAs https://app.dimensions.ai/details/grant/grant.3777594
88 schema:sdDatePublished 2019-03-07T11:22
89 schema:sdLicense https://scigraph.springernature.com/explorer/license/
90 schema:sdPublisher N1fd2e01a28ae470eaded0fdeca23a1cc
91 schema:startDate 2009-03-01T00:00:00Z
92 schema:url http://cordis.europa.eu/project/rcn/90072_en.html
93 sgo:license sg:explorer/license/
94 sgo:sdDataset grants
95 rdf:type schema:MonetaryGrant
96 N038c3bb8d04f49b2a219b68cc7807095 schema:member sg:person.01244052500.89
97 schema:roleName PI
98 rdf:type schema:Role
99 N07dac15a077d47aa9a94fafefe5fe0e0 schema:name dimensions_id
100 schema:value 3777594
101 rdf:type schema:PropertyValue
102 N1fd2e01a28ae470eaded0fdeca23a1cc schema:name Springer Nature - SN SciGraph project
103 rdf:type schema:Organization
104 N357c8a06878749fb9b579cac7bd640f9 schema:name cordis_id
105 schema:value 90072
106 rdf:type schema:PropertyValue
107 N5b06381b5ecd4ceb8f9c899ff893bd19 schema:currency EUR
108 schema:value 2106000
109 rdf:type schema:MonetaryAmount
110 anzsrc-for:2206 schema:inDefinedTermSet anzsrc-for:
111 rdf:type schema:DefinedTerm
112 sg:person.01244052500.89 schema:affiliation https://www.grid.ac/institutes/grid.418101.d
113 schema:familyName Clevers
114 schema:givenName Johannes Carolus
115 rdf:type schema:Person
116 https://www.grid.ac/institutes/grid.418101.d schema:name KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPEN - KNAW
117 rdf:type schema:Organization
118 https://www.grid.ac/institutes/grid.452896.4 schema:Organization
 




Preview window. Press ESC to close (or click here)


...