Chemical Biology Analysis of RNA Splicing Inhibitors.


Ontology type: schema:MonetaryGrant     


Grant Info

YEARS

2013-2015

FUNDING AMOUNT

44399 GBP

ABSTRACT

My research programme is aimed at understanding how the nucleus is organised in mammalian cells, how specific subnuclear bodies are assembled and regulated and how nuclear structure is related to biological function and to human molecular disease. A major theme of the programme is to use a combination of quantitative in vivo and in vitro techniques to measure dynamic processes in the cell nucleus. To achieve these aims we will focus primarily on studying the dynamic properties of the nucleolus and the nuclear organisation and function of the pre-mRNA splicing machinery. We will also study the role of reversible protein phosphorylation in regulating both nucleolar interactions and the splicing mechanism. We will compare data derived from biochemical experiments carried out using nuclear extracts prepared from cultured cell lines with data on the movement and interactions of proteins in vivo in the same cell lines. We will use proteomic methods to analyse and quantitate the protein composition of nucleoli under different growth and metabolic conditions and we will characterise dynamic interactions in live cells by imaging Fluorescence Protein-tagged fusion proteins. In parallel, we will analyse the organisation and structure of nuclear factors in both transformed and normal human tissue, derived from patient biopsies, to maximise the opportunities for our work on the cell biology of the nucleus to enhance clinical practice. More... »

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2206", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "amount": {
      "currency": "GBP", 
      "type": "MonetaryAmount", 
      "value": "44399"
    }, 
    "description": "My research programme is aimed at understanding how the nucleus is organised in mammalian cells, how specific subnuclear bodies are assembled and regulated and how nuclear structure is related to biological function and to human molecular disease. A major theme of the programme is to use a combination of quantitative in vivo and in vitro techniques to measure dynamic processes in the cell nucleus. To achieve these aims we will focus primarily on studying the dynamic properties of the nucleolus and the nuclear organisation and function of the pre-mRNA splicing machinery. We will also study the role of reversible protein phosphorylation in regulating both nucleolar interactions and the splicing mechanism. We will compare data derived from biochemical experiments carried out using nuclear extracts prepared from cultured cell lines with data on the movement and interactions of proteins in vivo in the same cell lines. We will use proteomic methods to analyse and quantitate the protein composition of nucleoli under different growth and metabolic conditions and we will characterise dynamic interactions in live cells by imaging Fluorescence Protein-tagged fusion proteins. In parallel, we will analyse the organisation and structure of nuclear factors in both transformed and normal human tissue, derived from patient biopsies, to maximise the opportunities for our work on the cell biology of the nucleus to enhance clinical practice.", 
    "endDate": "2015-09-30T00:00:00Z", 
    "funder": {
      "id": "https://www.grid.ac/institutes/grid.52788.30", 
      "type": "Organization"
    }, 
    "id": "sg:grant.3632288", 
    "identifier": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "3632288"
        ]
      }, 
      {
        "name": "wt_id", 
        "type": "PropertyValue", 
        "value": [
          "073980/Z/03/D"
        ]
      }
    ], 
    "inLanguage": [
      "en"
    ], 
    "keywords": [
      "same cell line", 
      "specific subnuclear bodies", 
      "organization", 
      "data", 
      "Chemical Biology Analysis", 
      "nuclear factor", 
      "opportunity", 
      "structure", 
      "dynamic process", 
      "major themes", 
      "cell biology", 
      "nucleus", 
      "protein", 
      "movement", 
      "human molecular disease", 
      "fusion protein", 
      "reversible protein phosphorylation", 
      "nuclear extracts", 
      "biological functions", 
      "nucleoli", 
      "research program", 
      "vitro techniques", 
      "parallel", 
      "cell nucleus", 
      "proteomic methods", 
      "interaction", 
      "different growth", 
      "vivo", 
      "function", 
      "metabolic conditions", 
      "nuclear organization", 
      "aim", 
      "cultured cell lines", 
      "splicing mechanism", 
      "RNA Splicing Inhibitors", 
      "pre-mRNA splicing machinery", 
      "mammalian cells", 
      "biochemical experiments", 
      "protein composition", 
      "nucleolus", 
      "patient biopsies", 
      "nuclear structure", 
      "combination", 
      "dynamic interaction", 
      "work", 
      "program", 
      "clinical practice", 
      "normal human tissues", 
      "fluorescence protein", 
      "nucleolar interactions", 
      "live cells", 
      "dynamic properties", 
      "role"
    ], 
    "name": "Chemical Biology Analysis of RNA Splicing Inhibitors.", 
    "recipient": [
      {
        "id": "https://www.grid.ac/institutes/grid.8241.f", 
        "type": "Organization"
      }, 
      {
        "affiliation": {
          "id": "https://www.grid.ac/institutes/grid.8241.f", 
          "name": "University of Dundee", 
          "type": "Organization"
        }, 
        "familyName": "Lamond", 
        "givenName": "Angus", 
        "id": "sg:person.0730433357.92", 
        "type": "Person"
      }, 
      {
        "member": "sg:person.0730433357.92", 
        "roleName": "PI", 
        "type": "Role"
      }
    ], 
    "sameAs": [
      "https://app.dimensions.ai/details/grant/grant.3632288"
    ], 
    "sdDataset": "grants", 
    "sdDatePublished": "2019-03-07T13:01", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com.uberresearch.data.processor/core_data/20181219_192338/projects/base/wt_projects.xml.gz", 
    "startDate": "2013-09-01T00:00:00Z", 
    "type": "MonetaryGrant"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/grant.3632288'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/grant.3632288'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/grant.3632288'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/grant.3632288'


 

This table displays all metadata directly associated to this object as RDF triples.

96 TRIPLES      18 PREDICATES      74 URIs      66 LITERALS      5 BLANK NODES

Subject Predicate Object
1 sg:grant.3632288 schema:about anzsrc-for:2206
2 schema:amount N03f950cac8cd457eac7d26917adf9ed3
3 schema:description My research programme is aimed at understanding how the nucleus is organised in mammalian cells, how specific subnuclear bodies are assembled and regulated and how nuclear structure is related to biological function and to human molecular disease. A major theme of the programme is to use a combination of quantitative in vivo and in vitro techniques to measure dynamic processes in the cell nucleus. To achieve these aims we will focus primarily on studying the dynamic properties of the nucleolus and the nuclear organisation and function of the pre-mRNA splicing machinery. We will also study the role of reversible protein phosphorylation in regulating both nucleolar interactions and the splicing mechanism. We will compare data derived from biochemical experiments carried out using nuclear extracts prepared from cultured cell lines with data on the movement and interactions of proteins in vivo in the same cell lines. We will use proteomic methods to analyse and quantitate the protein composition of nucleoli under different growth and metabolic conditions and we will characterise dynamic interactions in live cells by imaging Fluorescence Protein-tagged fusion proteins. In parallel, we will analyse the organisation and structure of nuclear factors in both transformed and normal human tissue, derived from patient biopsies, to maximise the opportunities for our work on the cell biology of the nucleus to enhance clinical practice.
4 schema:endDate 2015-09-30T00:00:00Z
5 schema:funder https://www.grid.ac/institutes/grid.52788.30
6 schema:identifier Nab674872e97347e095da8b864acd39f9
7 Nb07d6401ab3f4ce8a17f6dd4730e7cc3
8 schema:inLanguage en
9 schema:keywords Chemical Biology Analysis
10 RNA Splicing Inhibitors
11 aim
12 biochemical experiments
13 biological functions
14 cell biology
15 cell nucleus
16 clinical practice
17 combination
18 cultured cell lines
19 data
20 different growth
21 dynamic interaction
22 dynamic process
23 dynamic properties
24 fluorescence protein
25 function
26 fusion protein
27 human molecular disease
28 interaction
29 live cells
30 major themes
31 mammalian cells
32 metabolic conditions
33 movement
34 normal human tissues
35 nuclear extracts
36 nuclear factor
37 nuclear organization
38 nuclear structure
39 nucleolar interactions
40 nucleoli
41 nucleolus
42 nucleus
43 opportunity
44 organization
45 parallel
46 patient biopsies
47 pre-mRNA splicing machinery
48 program
49 protein
50 protein composition
51 proteomic methods
52 research program
53 reversible protein phosphorylation
54 role
55 same cell line
56 specific subnuclear bodies
57 splicing mechanism
58 structure
59 vitro techniques
60 vivo
61 work
62 schema:name Chemical Biology Analysis of RNA Splicing Inhibitors.
63 schema:recipient N38099ac357324de09dc2ea59d832cbb9
64 sg:person.0730433357.92
65 https://www.grid.ac/institutes/grid.8241.f
66 schema:sameAs https://app.dimensions.ai/details/grant/grant.3632288
67 schema:sdDatePublished 2019-03-07T13:01
68 schema:sdLicense https://scigraph.springernature.com/explorer/license/
69 schema:sdPublisher N62a158b0ab5e4fa98bfe2673533abc7c
70 schema:startDate 2013-09-01T00:00:00Z
71 sgo:license sg:explorer/license/
72 sgo:sdDataset grants
73 rdf:type schema:MonetaryGrant
74 N03f950cac8cd457eac7d26917adf9ed3 schema:currency GBP
75 schema:value 44399
76 rdf:type schema:MonetaryAmount
77 N38099ac357324de09dc2ea59d832cbb9 schema:member sg:person.0730433357.92
78 schema:roleName PI
79 rdf:type schema:Role
80 N62a158b0ab5e4fa98bfe2673533abc7c schema:name Springer Nature - SN SciGraph project
81 rdf:type schema:Organization
82 Nab674872e97347e095da8b864acd39f9 schema:name wt_id
83 schema:value 073980/Z/03/D
84 rdf:type schema:PropertyValue
85 Nb07d6401ab3f4ce8a17f6dd4730e7cc3 schema:name dimensions_id
86 schema:value 3632288
87 rdf:type schema:PropertyValue
88 anzsrc-for:2206 schema:inDefinedTermSet anzsrc-for:
89 rdf:type schema:DefinedTerm
90 sg:person.0730433357.92 schema:affiliation https://www.grid.ac/institutes/grid.8241.f
91 schema:familyName Lamond
92 schema:givenName Angus
93 rdf:type schema:Person
94 https://www.grid.ac/institutes/grid.52788.30 schema:Organization
95 https://www.grid.ac/institutes/grid.8241.f schema:name University of Dundee
96 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...