Genetics Of The C Elegans Dauer Larva View Homepage


Ontology type: schema:MonetaryGrant     


Grant Info

YEARS

1977-1999

FUNDING AMOUNT

2236735.0 USD

ABSTRACT

The goal is to determine the molecular and genetic mechanisms controlling a developmental "switch" in a simple animal model. The approach utilizes the advantages that the nematode C. elegans offers for molecular genetic studies. The switch is manifested at the second larval molt when alternate developmental fates may be expressed (either formation of a growing third-stage larva, or formation of a developmentally arrested, non-feeding dauer larva) based on environmental conditions. Starvation and overcrowding during the first larval stage induces formation of dauer larvae, which may survive for months, and when they find conditions favorable for growth, they resume development. The developmental decisions governing entry into, or exit from, the dauer stage are made in response to the ratio of food to the C. elegans dauer-inducing pheromone. Mutants affected in the decision to form dauer larvae are either dauer- constitutive, which form dauer larvae in abundant food, or dauer- defective, which cannot form dauer larvae when starved. Interactions between specific mutants have been used to construct pathways for gene action, now including more than 20 genes. Three genes have been cloned by transposon-tagging, and they encode molecules. Intermediate steps in the pathway are mediated by the daf-1 and daf-4 genes, which encode transmembrane receptor serine kinases. The daf-1 receptor was the first receptor serine kinase reported, and mammalian activin and TGF-beta receptors subsequently were found to be related to it. Activin and TGF- beta are growth factors of profound importance in vertebrate development. If the signal transduction pathway that regulates the C. elegans dauer larva is the nematode analogue of a TGF-beta or activin signalling system, C. elegans genetics may provide the means to identify the missing links between signal generation and control of gene expression in these vertebrate systems. The daf-12 gene specifies what we believe to be the last step in signal transduction. It encodes a member of the steroid-thyroid hormone receptor superfamily. Whereas daf-1 and daf-4 are required for normal non-dauer development, daf-12 activity is required for dauer larva morphogenesis. We propose that the daf kinases phosphorylate proteins that promote growth, and directly or indirectly inactivate the daf-12 receptor, possibly by preventing synthesis of a dauer-inducing hormone. Specific goals fall into complementary areas aimed at (1) understanding the relationship between the dauer and activin/TGF-beta signalling system, (2) identifying the ligands for the daf-1 and daf-4 receptors, (3) identifying the downstream targets for phosphorylation, (4) understanding how mutational changes affect daf-1 and 4 structure and function, (5) determining whether the daf-12 receptor is a ligand- activated transcription factor, (6) identifying DNA target sites for daf- 12 action, (7) genetically identifying additional genes involved in the dauer pathway, including those which may have essential functions in development, and (8) characterizing daf gene homologs in a parasitic nematode. More... »

URL

http://projectreporter.nih.gov/project_info_description.cfm?aid=2403064

Related SciGraph Publications

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "amount": {
      "currency": "USD", 
      "type": "MonetaryAmount", 
      "value": 2236735.0
    }, 
    "description": "The goal is to determine the molecular and genetic mechanisms controlling a developmental \"switch\" in a simple animal model. The approach utilizes the advantages that the nematode C. elegans offers for molecular genetic studies. The switch is manifested at the second larval molt when alternate developmental fates may be expressed (either formation of a growing third-stage larva, or formation of a developmentally arrested, non-feeding dauer larva) based on environmental conditions. Starvation and overcrowding during the first larval stage induces formation of dauer larvae, which may survive for months, and when they find conditions favorable for growth, they resume development. The developmental decisions governing entry into, or exit from, the dauer stage are made in response to the ratio of food to the C. elegans dauer-inducing pheromone. Mutants affected in the decision to form dauer larvae are either dauer- constitutive, which form dauer larvae in abundant food, or dauer- defective, which cannot form dauer larvae when starved. Interactions between specific mutants have been used to construct pathways for gene action, now including more than 20 genes. Three genes have been cloned by transposon-tagging, and they encode molecules. Intermediate steps in the pathway are mediated by the daf-1 and daf-4 genes, which encode transmembrane receptor serine kinases. The daf-1 receptor was the first receptor serine kinase reported, and mammalian activin and TGF-beta receptors subsequently were found to be related to it. Activin and TGF- beta are growth factors of profound importance in vertebrate development. If the signal transduction pathway that regulates the C. elegans dauer larva is the nematode analogue of a TGF-beta or activin signalling system, C. elegans genetics may provide the means to identify the missing links between signal generation and control of gene expression in these vertebrate systems. The daf-12 gene specifies what we believe to be the last step in signal transduction. It encodes a member of the steroid-thyroid hormone receptor superfamily. Whereas daf-1 and daf-4 are required for normal non-dauer development, daf-12 activity is required for dauer larva morphogenesis. We propose that the daf kinases phosphorylate proteins that promote growth, and directly or indirectly inactivate the daf-12 receptor, possibly by preventing synthesis of a dauer-inducing hormone. Specific goals fall into complementary areas aimed at (1) understanding the relationship between the dauer and activin/TGF-beta signalling system, (2) identifying the ligands for the daf-1 and daf-4 receptors, (3) identifying the downstream targets for phosphorylation, (4) understanding how mutational changes affect daf-1 and 4 structure and function, (5) determining whether the daf-12 receptor is a ligand- activated transcription factor, (6) identifying DNA target sites for daf- 12 action, (7) genetically identifying additional genes involved in the dauer pathway, including those which may have essential functions in development, and (8) characterizing daf gene homologs in a parasitic nematode.", 
    "endDate": "1999-03-31", 
    "funder": {
      "id": "http://www.grid.ac/institutes/grid.420089.7", 
      "type": "Organization"
    }, 
    "id": "sg:grant.2522407", 
    "identifier": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "grant.2522407"
        ]
      }, 
      {
        "name": "nih_id", 
        "type": "PropertyValue", 
        "value": [
          "R01HD011239"
        ]
      }
    ], 
    "keywords": [
      "receptor serine kinase", 
      "dauer larvae", 
      "DAF-1", 
      "DAF-12 receptor", 
      "serine kinase", 
      "C. elegans", 
      "non-dauer development", 
      "C. elegans genetics", 
      "steroid-thyroid hormone receptor", 
      "second larval molt", 
      "nematode C. elegans", 
      "DNA target sites", 
      "signal transduction pathways", 
      "dauer-inducing pheromone", 
      "first larval stage", 
      "dauer pathway", 
      "molecular genetic studies", 
      "phosphorylate proteins", 
      "vertebrate development", 
      "gene specifies", 
      "developmental decisions", 
      "gene homologs", 
      "dauer stage", 
      "daf-4", 
      "TGF-beta receptors", 
      "vertebrate systems", 
      "developmental fate", 
      "signal transduction", 
      "transcription factors", 
      "specific mutants", 
      "additional genes", 
      "transduction pathways", 
      "gene action", 
      "parasitic nematodes", 
      "genetic mechanisms", 
      "downstream targets", 
      "gene expression", 
      "mutational changes", 
      "essential functions", 
      "larval stages", 
      "abundant food", 
      "genetic studies", 
      "larval molt", 
      "genes", 
      "larvae", 
      "environmental conditions", 
      "elegans", 
      "target site", 
      "simple animal model", 
      "mutants", 
      "TGF beta", 
      "pathway", 
      "kinase", 
      "activin", 
      "hormone receptors", 
      "growth factor", 
      "receptors", 
      "dauer", 
      "last step", 
      "homolog", 
      "GENETICS", 
      "morphogenesis", 
      "transposon", 
      "complementary areas", 
      "transduction", 
      "phosphorylation", 
      "nematodes", 
      "genetics", 
      "profound importance", 
      "starvation", 
      "protein", 
      "growth", 
      "moult", 
      "pheromone", 
      "DAF", 
      "fate", 
      "expression", 
      "switch", 
      "intermediate step", 
      "animal models", 
      "function", 
      "development", 
      "members", 
      "stage", 
      "target", 
      "food", 
      "sites", 
      "molecules", 
      "mechanism", 
      "ligands", 
      "interaction", 
      "activity", 
      "action", 
      "factors", 
      "hormone", 
      "step", 
      "response", 
      "exit", 
      "entry", 
      "formation", 
      "synthesis", 
      "conditions", 
      "importance", 
      "structure", 
      "specifies", 
      "changes", 
      "analogues", 
      "signal generation", 
      "generation", 
      "link", 
      "system", 
      "control", 
      "relationship", 
      "study", 
      "area", 
      "approach", 
      "goal", 
      "ratio of food", 
      "specific goals", 
      "model", 
      "advantages", 
      "ratio", 
      "means", 
      "decisions", 
      "months"
    ], 
    "name": "GENETICS OF THE C ELEGANS DAUER LARVA", 
    "recipient": [
      {
        "id": "http://www.grid.ac/institutes/grid.134936.a", 
        "type": "Organization"
      }, 
      {
        "affiliation": {
          "id": "http://www.grid.ac/institutes/None", 
          "name": "UNIVERSITY OF MISSOURI-COLUMBIA", 
          "type": "Organization"
        }, 
        "familyName": "RIDDLE", 
        "givenName": "DONALD L.", 
        "id": "sg:person.0741240470.74", 
        "type": "Person"
      }, 
      {
        "member": "sg:person.0741240470.74", 
        "roleName": "PI", 
        "type": "Role"
      }
    ], 
    "sameAs": [
      "https://app.dimensions.ai/details/grant/grant.2522407"
    ], 
    "sdDataset": "grants", 
    "sdDatePublished": "2022-10-01T07:03", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221001/entities/gbq_results/grant/grant_67.jsonl", 
    "startDate": "1977-07-01", 
    "type": "MonetaryGrant", 
    "url": "http://projectreporter.nih.gov/project_info_description.cfm?aid=2403064"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/grant.2522407'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/grant.2522407'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/grant.2522407'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/grant.2522407'


 

This table displays all metadata directly associated to this object as RDF triples.

169 TRIPLES      18 PREDICATES      146 URIs      138 LITERALS      5 BLANK NODES

Subject Predicate Object
1 sg:grant.2522407 schema:about anzsrc-for:06
2 schema:amount N7b56e8faddb0494cae934e22751fbf39
3 schema:description The goal is to determine the molecular and genetic mechanisms controlling a developmental "switch" in a simple animal model. The approach utilizes the advantages that the nematode C. elegans offers for molecular genetic studies. The switch is manifested at the second larval molt when alternate developmental fates may be expressed (either formation of a growing third-stage larva, or formation of a developmentally arrested, non-feeding dauer larva) based on environmental conditions. Starvation and overcrowding during the first larval stage induces formation of dauer larvae, which may survive for months, and when they find conditions favorable for growth, they resume development. The developmental decisions governing entry into, or exit from, the dauer stage are made in response to the ratio of food to the C. elegans dauer-inducing pheromone. Mutants affected in the decision to form dauer larvae are either dauer- constitutive, which form dauer larvae in abundant food, or dauer- defective, which cannot form dauer larvae when starved. Interactions between specific mutants have been used to construct pathways for gene action, now including more than 20 genes. Three genes have been cloned by transposon-tagging, and they encode molecules. Intermediate steps in the pathway are mediated by the daf-1 and daf-4 genes, which encode transmembrane receptor serine kinases. The daf-1 receptor was the first receptor serine kinase reported, and mammalian activin and TGF-beta receptors subsequently were found to be related to it. Activin and TGF- beta are growth factors of profound importance in vertebrate development. If the signal transduction pathway that regulates the C. elegans dauer larva is the nematode analogue of a TGF-beta or activin signalling system, C. elegans genetics may provide the means to identify the missing links between signal generation and control of gene expression in these vertebrate systems. The daf-12 gene specifies what we believe to be the last step in signal transduction. It encodes a member of the steroid-thyroid hormone receptor superfamily. Whereas daf-1 and daf-4 are required for normal non-dauer development, daf-12 activity is required for dauer larva morphogenesis. We propose that the daf kinases phosphorylate proteins that promote growth, and directly or indirectly inactivate the daf-12 receptor, possibly by preventing synthesis of a dauer-inducing hormone. Specific goals fall into complementary areas aimed at (1) understanding the relationship between the dauer and activin/TGF-beta signalling system, (2) identifying the ligands for the daf-1 and daf-4 receptors, (3) identifying the downstream targets for phosphorylation, (4) understanding how mutational changes affect daf-1 and 4 structure and function, (5) determining whether the daf-12 receptor is a ligand- activated transcription factor, (6) identifying DNA target sites for daf- 12 action, (7) genetically identifying additional genes involved in the dauer pathway, including those which may have essential functions in development, and (8) characterizing daf gene homologs in a parasitic nematode.
4 schema:endDate 1999-03-31
5 schema:funder grid-institutes:grid.420089.7
6 schema:identifier N525c5cd95dd04b2a9d5e90f29ca7a806
7 N58e994278d3846c9a551672781ff6214
8 schema:keywords C. elegans
9 C. elegans genetics
10 DAF
11 DAF-1
12 DAF-12 receptor
13 DNA target sites
14 GENETICS
15 TGF beta
16 TGF-beta receptors
17 abundant food
18 action
19 activin
20 activity
21 additional genes
22 advantages
23 analogues
24 animal models
25 approach
26 area
27 changes
28 complementary areas
29 conditions
30 control
31 daf-4
32 dauer
33 dauer larvae
34 dauer pathway
35 dauer stage
36 dauer-inducing pheromone
37 decisions
38 development
39 developmental decisions
40 developmental fate
41 downstream targets
42 elegans
43 entry
44 environmental conditions
45 essential functions
46 exit
47 expression
48 factors
49 fate
50 first larval stage
51 food
52 formation
53 function
54 gene action
55 gene expression
56 gene homologs
57 gene specifies
58 generation
59 genes
60 genetic mechanisms
61 genetic studies
62 genetics
63 goal
64 growth
65 growth factor
66 homolog
67 hormone
68 hormone receptors
69 importance
70 interaction
71 intermediate step
72 kinase
73 larvae
74 larval molt
75 larval stages
76 last step
77 ligands
78 link
79 means
80 mechanism
81 members
82 model
83 molecular genetic studies
84 molecules
85 months
86 morphogenesis
87 moult
88 mutants
89 mutational changes
90 nematode C. elegans
91 nematodes
92 non-dauer development
93 parasitic nematodes
94 pathway
95 pheromone
96 phosphorylate proteins
97 phosphorylation
98 profound importance
99 protein
100 ratio
101 ratio of food
102 receptor serine kinase
103 receptors
104 relationship
105 response
106 second larval molt
107 serine kinase
108 signal generation
109 signal transduction
110 signal transduction pathways
111 simple animal model
112 sites
113 specific goals
114 specific mutants
115 specifies
116 stage
117 starvation
118 step
119 steroid-thyroid hormone receptor
120 structure
121 study
122 switch
123 synthesis
124 system
125 target
126 target site
127 transcription factors
128 transduction
129 transduction pathways
130 transposon
131 vertebrate development
132 vertebrate systems
133 schema:name GENETICS OF THE C ELEGANS DAUER LARVA
134 schema:recipient N2f34e78018a848c7a7502f2fc02d808c
135 sg:person.0741240470.74
136 grid-institutes:grid.134936.a
137 schema:sameAs https://app.dimensions.ai/details/grant/grant.2522407
138 schema:sdDatePublished 2022-10-01T07:03
139 schema:sdLicense https://scigraph.springernature.com/explorer/license/
140 schema:sdPublisher Nd02fd4441e384d1d923629829371a541
141 schema:startDate 1977-07-01
142 schema:url http://projectreporter.nih.gov/project_info_description.cfm?aid=2403064
143 sgo:license sg:explorer/license/
144 sgo:sdDataset grants
145 rdf:type schema:MonetaryGrant
146 N2f34e78018a848c7a7502f2fc02d808c schema:member sg:person.0741240470.74
147 schema:roleName PI
148 rdf:type schema:Role
149 N525c5cd95dd04b2a9d5e90f29ca7a806 schema:name dimensions_id
150 schema:value grant.2522407
151 rdf:type schema:PropertyValue
152 N58e994278d3846c9a551672781ff6214 schema:name nih_id
153 schema:value R01HD011239
154 rdf:type schema:PropertyValue
155 N7b56e8faddb0494cae934e22751fbf39 schema:currency USD
156 schema:value 2236735.0
157 rdf:type schema:MonetaryAmount
158 Nd02fd4441e384d1d923629829371a541 schema:name Springer Nature - SN SciGraph project
159 rdf:type schema:Organization
160 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
161 rdf:type schema:DefinedTerm
162 sg:person.0741240470.74 schema:affiliation grid-institutes:None
163 schema:familyName RIDDLE
164 schema:givenName DONALD L.
165 rdf:type schema:Person
166 grid-institutes:None schema:name UNIVERSITY OF MISSOURI-COLUMBIA
167 rdf:type schema:Organization
168 grid-institutes:grid.134936.a schema:Organization
169 grid-institutes:grid.420089.7 schema:Organization
 




Preview window. Press ESC to close (or click here)


...