Improved Adenoviral Vectors for Hepatic Gene Therapy View Homepage


Ontology type: schema:MonetaryGrant     


Grant Info

YEARS

1994-2007

FUNDING AMOUNT

1884434 USD

ABSTRACT

DESCRIPTION (provided by applicant): Recombinant adenoviral vectors have had a stormy history. Nevertheless, there is promise in the development of gene-deleted adenoviruses because of reduced toxicity, efficient gene transfer, and large DNA carrying capacity. The goal of this project is to develop scientific principles required for production and evaluation of gene-deleted adenoviral vectors that remain episomal and/or integrate into host chromosomal DNA. The new vectors will be assessed in animals for efficacy as well as safety with the primary focus being on liver gene transfer. Using DNA transposons and site-specific phage integrases, we plan to develop gone-deleted vectors that can integrate an expression cassette into the host chromosome. Both episomal and integrating gene deleted vectors will be compared for longevity of therapeutic gene expression in a dog model of hemophilia. This will allow us to establish the utility of use of the vector for treating genetic diseases where life-long gene expression is required in most situations. We will begin to attempt to unravel the molecular state of the vector DNA and identify cellular proteins that may be involved in stabilizing episomal adenoviral vector DNAs in vivo. Taken together, these studies will advance our basic understanding of vector-host interactions related to persistence of vector in rive, as well as advancing therapeutic applications in preclinical development. More... »

URL

http://projectreporter.nih.gov/project_info_description.cfm?aid=7028393

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2206", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "amount": {
      "currency": "USD", 
      "type": "MonetaryAmount", 
      "value": "1884434"
    }, 
    "description": "DESCRIPTION (provided by applicant): Recombinant adenoviral vectors have had a stormy history. Nevertheless, there is promise in the development of gene-deleted adenoviruses because of reduced toxicity, efficient gene transfer, and large DNA carrying capacity. The goal of this project is to develop scientific principles required for production and evaluation of gene-deleted adenoviral vectors that remain episomal and/or integrate into host chromosomal DNA. The new vectors will be assessed in animals for efficacy as well as safety with the primary focus being on liver gene transfer. Using DNA transposons and site-specific phage integrases, we plan to develop gone-deleted vectors that can integrate an expression cassette into the host chromosome. Both episomal and integrating gene deleted vectors will be compared for longevity of therapeutic gene expression in a dog model of hemophilia. This will allow us to establish the utility of use of the vector for treating genetic diseases where life-long gene expression is required in most situations. We will begin to attempt to unravel the molecular state of the vector DNA and identify cellular proteins that may be involved in stabilizing episomal adenoviral vector DNAs in vivo. Taken together, these studies will advance our basic understanding of vector-host interactions related to persistence of vector in rive, as well as advancing therapeutic applications in preclinical development.", 
    "endDate": "2007-03-31T00:00:00Z", 
    "funder": {
      "id": "https://www.grid.ac/institutes/grid.419635.c", 
      "type": "Organization"
    }, 
    "id": "sg:grant.2495350", 
    "identifier": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "2495350"
        ]
      }, 
      {
        "name": "nih_id", 
        "type": "PropertyValue", 
        "value": [
          "R01DK049022"
        ]
      }
    ], 
    "inLanguage": [
      "en"
    ], 
    "keywords": [
      "primary focus", 
      "use", 
      "project", 
      "vivo", 
      "toxicity", 
      "promise", 
      "goal", 
      "molecular states", 
      "adenovirus", 
      "large DNA", 
      "genes", 
      "most situations", 
      "efficient gene transfer", 
      "development", 
      "therapeutic applications", 
      "life-long gene expression", 
      "adenoviral vector", 
      "evaluation", 
      "study", 
      "utility", 
      "genetic diseases", 
      "basic understanding", 
      "host", 
      "hemophilia", 
      "host chromosome", 
      "liver gene transfer", 
      "cellular proteins", 
      "vector", 
      "capacity", 
      "vector DNA", 
      "description", 
      "applicants", 
      "Rive", 
      "gone-deleted vectors", 
      "chromosomal DNA", 
      "scientific principles", 
      "persistence", 
      "Adenoviral Vectors", 
      "dog model", 
      "hepatic gene therapy", 
      "therapeutic gene expression", 
      "preclinical development", 
      "safety", 
      "production", 
      "animals", 
      "stormy history", 
      "site-specific phage integrases", 
      "episomal", 
      "expression cassette", 
      "longevity", 
      "episomal adenoviral vector DNAs", 
      "DNA transposons", 
      "new vector", 
      "efficacy", 
      "recombinant adenoviral vector", 
      "vector-host interactions"
    ], 
    "name": "Improved Adenoviral Vectors for Hepatic Gene Therapy", 
    "recipient": [
      {
        "id": "https://www.grid.ac/institutes/grid.168010.e", 
        "type": "Organization"
      }, 
      {
        "affiliation": {
          "id": "https://www.grid.ac/institutes/grid.168010.e", 
          "name": "STANFORD UNIVERSITY", 
          "type": "Organization"
        }, 
        "familyName": "KAY", 
        "givenName": "MARK A", 
        "id": "sg:person.01164322460.34", 
        "type": "Person"
      }, 
      {
        "member": "sg:person.01164322460.34", 
        "roleName": "PI", 
        "type": "Role"
      }
    ], 
    "sameAs": [
      "https://app.dimensions.ai/details/grant/grant.2495350"
    ], 
    "sdDataset": "grants", 
    "sdDatePublished": "2019-03-07T12:15", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com.uberresearch.data.processor/core_data/20181219_192338/projects/base/nih_projects_8.xml.gz", 
    "startDate": "1994-09-30T00:00:00Z", 
    "type": "MonetaryGrant", 
    "url": "http://projectreporter.nih.gov/project_info_description.cfm?aid=7028393"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/grant.2495350'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/grant.2495350'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/grant.2495350'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/grant.2495350'


 

This table displays all metadata directly associated to this object as RDF triples.

100 TRIPLES      19 PREDICATES      78 URIs      70 LITERALS      5 BLANK NODES

Subject Predicate Object
1 sg:grant.2495350 schema:about anzsrc-for:2206
2 schema:amount Nf87dc6e9f5744830b802432e12fe418e
3 schema:description DESCRIPTION (provided by applicant): Recombinant adenoviral vectors have had a stormy history. Nevertheless, there is promise in the development of gene-deleted adenoviruses because of reduced toxicity, efficient gene transfer, and large DNA carrying capacity. The goal of this project is to develop scientific principles required for production and evaluation of gene-deleted adenoviral vectors that remain episomal and/or integrate into host chromosomal DNA. The new vectors will be assessed in animals for efficacy as well as safety with the primary focus being on liver gene transfer. Using DNA transposons and site-specific phage integrases, we plan to develop gone-deleted vectors that can integrate an expression cassette into the host chromosome. Both episomal and integrating gene deleted vectors will be compared for longevity of therapeutic gene expression in a dog model of hemophilia. This will allow us to establish the utility of use of the vector for treating genetic diseases where life-long gene expression is required in most situations. We will begin to attempt to unravel the molecular state of the vector DNA and identify cellular proteins that may be involved in stabilizing episomal adenoviral vector DNAs in vivo. Taken together, these studies will advance our basic understanding of vector-host interactions related to persistence of vector in rive, as well as advancing therapeutic applications in preclinical development.
4 schema:endDate 2007-03-31T00:00:00Z
5 schema:funder https://www.grid.ac/institutes/grid.419635.c
6 schema:identifier N123e2b6c58a04fd8b6e212b3d99ec14e
7 Nfe2de6281ad74e1e809ffc2d01328bf4
8 schema:inLanguage en
9 schema:keywords Adenoviral Vectors
10 DNA transposons
11 Rive
12 adenoviral vector
13 adenovirus
14 animals
15 applicants
16 basic understanding
17 capacity
18 cellular proteins
19 chromosomal DNA
20 description
21 development
22 dog model
23 efficacy
24 efficient gene transfer
25 episomal
26 episomal adenoviral vector DNAs
27 evaluation
28 expression cassette
29 genes
30 genetic diseases
31 goal
32 gone-deleted vectors
33 hemophilia
34 hepatic gene therapy
35 host
36 host chromosome
37 large DNA
38 life-long gene expression
39 liver gene transfer
40 longevity
41 molecular states
42 most situations
43 new vector
44 persistence
45 preclinical development
46 primary focus
47 production
48 project
49 promise
50 recombinant adenoviral vector
51 safety
52 scientific principles
53 site-specific phage integrases
54 stormy history
55 study
56 therapeutic applications
57 therapeutic gene expression
58 toxicity
59 use
60 utility
61 vector
62 vector DNA
63 vector-host interactions
64 vivo
65 schema:name Improved Adenoviral Vectors for Hepatic Gene Therapy
66 schema:recipient N526a7ee1d1d445f29b39ee1abedf30bb
67 sg:person.01164322460.34
68 https://www.grid.ac/institutes/grid.168010.e
69 schema:sameAs https://app.dimensions.ai/details/grant/grant.2495350
70 schema:sdDatePublished 2019-03-07T12:15
71 schema:sdLicense https://scigraph.springernature.com/explorer/license/
72 schema:sdPublisher Nd2e970c621e047208d86363c8b39ad73
73 schema:startDate 1994-09-30T00:00:00Z
74 schema:url http://projectreporter.nih.gov/project_info_description.cfm?aid=7028393
75 sgo:license sg:explorer/license/
76 sgo:sdDataset grants
77 rdf:type schema:MonetaryGrant
78 N123e2b6c58a04fd8b6e212b3d99ec14e schema:name dimensions_id
79 schema:value 2495350
80 rdf:type schema:PropertyValue
81 N526a7ee1d1d445f29b39ee1abedf30bb schema:member sg:person.01164322460.34
82 schema:roleName PI
83 rdf:type schema:Role
84 Nd2e970c621e047208d86363c8b39ad73 schema:name Springer Nature - SN SciGraph project
85 rdf:type schema:Organization
86 Nf87dc6e9f5744830b802432e12fe418e schema:currency USD
87 schema:value 1884434
88 rdf:type schema:MonetaryAmount
89 Nfe2de6281ad74e1e809ffc2d01328bf4 schema:name nih_id
90 schema:value R01DK049022
91 rdf:type schema:PropertyValue
92 anzsrc-for:2206 schema:inDefinedTermSet anzsrc-for:
93 rdf:type schema:DefinedTerm
94 sg:person.01164322460.34 schema:affiliation https://www.grid.ac/institutes/grid.168010.e
95 schema:familyName KAY
96 schema:givenName MARK A
97 rdf:type schema:Person
98 https://www.grid.ac/institutes/grid.168010.e schema:name STANFORD UNIVERSITY
99 rdf:type schema:Organization
100 https://www.grid.ac/institutes/grid.419635.c schema:Organization
 




Preview window. Press ESC to close (or click here)


...