Regulation Of Human Cytolytic T Lymphocytes View Homepage


Ontology type: schema:MonetaryGrant     


Grant Info

YEARS

1982-1991

FUNDING AMOUNT

0 USD

ABSTRACT

The ultimate goal of this research is to define the human cytolytic T lymphocyte (CTL) response to leukemia by utilizing purified and well-characterized antigens, cloned CTL lines, and monoclonal antibodies to functional T-cell subsets. In order to better understand the CTL response, it is necessary to define the antigens that trigger this response and to define the cell interactiona that regulate this response. Since CTLs appear in all cases studied to respond to integral membrane proteins (whether they be foreign MHC antigens or autologous MHC antigens in association with foreign antigens such as a virus), it has been difficult to define the antigens involved in CTL triggering. Initial studies in murine xenogeneic, allogeneic, and syngeneic CTL model systems demonstrate that H-2 and HLA antigens can be isolated from the cell surface and retain CTL stimulating activity. These results suggest that the antigenic requirements for the stimulation of human CTLs can be approached in a similar manner. The ability to obtain cloned CTL lines should allow one to define more precisely the specificity of CTLs. Thus, one can hope to determine what target antigens are recognized by monoclonal CTLs when triggered by well-defined antigens, i.e., purified MHC antigens or purified viral antigens plus MHC antigens inserted into artificial membranes or liposomes. The ability to obtain cloned helper T cells or suppressor T cells should also allow a more careful delineation of the cell-cell interactions that regulate the CTL response. These cloned functional T-cell lines may provide the appropriate reagents to raise antibodies to antigens specific for functional subsets of T cells. The availability of antibodies to human CTLs should subdivide the T-cell subset defined by OKT5 and OKT8 and allow a separation of CTLs from suppressor T cells. These new reagents may also allow us to isolate biochemically and define these cell surface antigens, possibly even resulting in the isolation and characterization of functional T-cell receptors. (LB) More... »

URL

http://projectreporter.nih.gov/project_info_description.cfm?aid=3171888

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2211", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "amount": {
      "currency": "USD", 
      "type": "MonetaryAmount", 
      "value": "0"
    }, 
    "description": "The ultimate goal of this research is to define the human cytolytic T lymphocyte (CTL) response to leukemia by utilizing purified and well-characterized antigens, cloned CTL lines, and monoclonal antibodies to functional T-cell subsets. In order to better understand the CTL response, it is necessary to define the antigens that trigger this response and to define the cell interactiona that regulate this response. Since CTLs appear in all cases studied to respond to integral membrane proteins (whether they be foreign MHC antigens or autologous MHC antigens in association with foreign antigens such as a virus), it has been difficult to define the antigens involved in CTL triggering. Initial studies in murine xenogeneic, allogeneic, and syngeneic CTL model systems demonstrate that H-2 and HLA antigens can be isolated from the cell surface and retain CTL stimulating activity. These results suggest that the antigenic requirements for the stimulation of human CTLs can be approached in a similar manner. The ability to obtain cloned CTL lines should allow one to define more precisely the specificity of CTLs. Thus, one can hope to determine what target antigens are recognized by monoclonal CTLs when triggered by well-defined antigens, i.e., purified MHC antigens or purified viral antigens plus MHC antigens inserted into artificial membranes or liposomes. The ability to obtain cloned helper T cells or suppressor T cells should also allow a more careful delineation of the cell-cell interactions that regulate the CTL response. These cloned functional T-cell lines may provide the appropriate reagents to raise antibodies to antigens specific for functional subsets of T cells. The availability of antibodies to human CTLs should subdivide the T-cell subset defined by OKT5 and OKT8 and allow a separation of CTLs from suppressor T cells. These new reagents may also allow us to isolate biochemically and define these cell surface antigens, possibly even resulting in the isolation and characterization of functional T-cell receptors. (LB)", 
    "endDate": "1991-05-31T00:00:00Z", 
    "funder": {
      "id": "https://www.grid.ac/institutes/grid.48336.3a", 
      "type": "Organization"
    }, 
    "id": "sg:grant.2467307", 
    "identifier": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "2467307"
        ]
      }, 
      {
        "name": "nih_id", 
        "type": "PropertyValue", 
        "value": [
          "R01CA034129"
        ]
      }
    ], 
    "inLanguage": [
      "en"
    ], 
    "keywords": [
      "ability", 
      "functional subsets", 
      "characterization", 
      "isolation", 
      "OKT8", 
      "cell subsets", 
      "ultimate goal", 
      "similar manner", 
      "allogeneic", 
      "H-2", 
      "careful delineation", 
      "regulation", 
      "foreign antigens", 
      "monoclonal antibodies", 
      "association", 
      "leukemia", 
      "cases", 
      "research", 
      "CTL responses", 
      "CTL lines", 
      "foreign MHC antigens", 
      "appropriate reagents", 
      "virus", 
      "cells", 
      "HUMAN CYTOLYTIC T LYMPHOCYTES", 
      "cell surface", 
      "CTL model systems", 
      "separation", 
      "new reagent", 
      "monoclonal CTL", 
      "autologous MHC antigens", 
      "artificial membranes", 
      "lb", 
      "results", 
      "human CTL", 
      "integral membrane proteins", 
      "target antigen", 
      "specificity", 
      "liposomes", 
      "activity", 
      "human cytolytic T lymphocytes", 
      "purified viral antigens", 
      "cell interactiona", 
      "cell surface antigens", 
      "stimulation", 
      "OKT5", 
      "murine xenogeneic", 
      "cell-cell interactions", 
      "initial study", 
      "cell lines", 
      "antibodies", 
      "response", 
      "antigenic requirements", 
      "HLA antigens", 
      "suppressor T cells", 
      "order", 
      "cell receptor", 
      "purified MHC antigens", 
      "MHC antigens", 
      "availability", 
      "antigen", 
      "helper T cells"
    ], 
    "name": "REGULATION OF HUMAN CYTOLYTIC T LYMPHOCYTES", 
    "recipient": [
      {
        "id": "https://www.grid.ac/institutes/grid.65499.37", 
        "type": "Organization"
      }, 
      {
        "affiliation": {
          "id": "https://www.grid.ac/institutes/grid.65499.37", 
          "name": "DANA-FARBER CANCER INSTITUTE", 
          "type": "Organization"
        }, 
        "familyName": "BURAKOFF", 
        "givenName": "STEVEN J.", 
        "id": "sg:person.01007001471.61", 
        "type": "Person"
      }, 
      {
        "member": "sg:person.01007001471.61", 
        "roleName": "PI", 
        "type": "Role"
      }
    ], 
    "sameAs": [
      "https://app.dimensions.ai/details/grant/grant.2467307"
    ], 
    "sdDataset": "grants", 
    "sdDatePublished": "2019-03-07T12:13", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com.uberresearch.data.processor/core_data/20181219_192338/projects/base/nih_projects_6.xml.gz", 
    "startDate": "1982-07-01T00:00:00Z", 
    "type": "MonetaryGrant", 
    "url": "http://projectreporter.nih.gov/project_info_description.cfm?aid=3171888"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/grant.2467307'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/grant.2467307'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/grant.2467307'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/grant.2467307'


 

This table displays all metadata directly associated to this object as RDF triples.

106 TRIPLES      19 PREDICATES      84 URIs      76 LITERALS      5 BLANK NODES

Subject Predicate Object
1 sg:grant.2467307 schema:about anzsrc-for:2211
2 schema:amount Na852e93fdd0d4bc0b1eb29435304eb94
3 schema:description The ultimate goal of this research is to define the human cytolytic T lymphocyte (CTL) response to leukemia by utilizing purified and well-characterized antigens, cloned CTL lines, and monoclonal antibodies to functional T-cell subsets. In order to better understand the CTL response, it is necessary to define the antigens that trigger this response and to define the cell interactiona that regulate this response. Since CTLs appear in all cases studied to respond to integral membrane proteins (whether they be foreign MHC antigens or autologous MHC antigens in association with foreign antigens such as a virus), it has been difficult to define the antigens involved in CTL triggering. Initial studies in murine xenogeneic, allogeneic, and syngeneic CTL model systems demonstrate that H-2 and HLA antigens can be isolated from the cell surface and retain CTL stimulating activity. These results suggest that the antigenic requirements for the stimulation of human CTLs can be approached in a similar manner. The ability to obtain cloned CTL lines should allow one to define more precisely the specificity of CTLs. Thus, one can hope to determine what target antigens are recognized by monoclonal CTLs when triggered by well-defined antigens, i.e., purified MHC antigens or purified viral antigens plus MHC antigens inserted into artificial membranes or liposomes. The ability to obtain cloned helper T cells or suppressor T cells should also allow a more careful delineation of the cell-cell interactions that regulate the CTL response. These cloned functional T-cell lines may provide the appropriate reagents to raise antibodies to antigens specific for functional subsets of T cells. The availability of antibodies to human CTLs should subdivide the T-cell subset defined by OKT5 and OKT8 and allow a separation of CTLs from suppressor T cells. These new reagents may also allow us to isolate biochemically and define these cell surface antigens, possibly even resulting in the isolation and characterization of functional T-cell receptors. (LB)
4 schema:endDate 1991-05-31T00:00:00Z
5 schema:funder https://www.grid.ac/institutes/grid.48336.3a
6 schema:identifier N3286ce0b115f4e8786cd1a6853fcd8c1
7 N7227c30ae7a94e26bca81d47d781a660
8 schema:inLanguage en
9 schema:keywords CTL lines
10 CTL model systems
11 CTL responses
12 H-2
13 HLA antigens
14 HUMAN CYTOLYTIC T LYMPHOCYTES
15 MHC antigens
16 OKT5
17 OKT8
18 ability
19 activity
20 allogeneic
21 antibodies
22 antigen
23 antigenic requirements
24 appropriate reagents
25 artificial membranes
26 association
27 autologous MHC antigens
28 availability
29 careful delineation
30 cases
31 cell interactiona
32 cell lines
33 cell receptor
34 cell subsets
35 cell surface
36 cell surface antigens
37 cell-cell interactions
38 cells
39 characterization
40 foreign MHC antigens
41 foreign antigens
42 functional subsets
43 helper T cells
44 human CTL
45 human cytolytic T lymphocytes
46 initial study
47 integral membrane proteins
48 isolation
49 lb
50 leukemia
51 liposomes
52 monoclonal CTL
53 monoclonal antibodies
54 murine xenogeneic
55 new reagent
56 order
57 purified MHC antigens
58 purified viral antigens
59 regulation
60 research
61 response
62 results
63 separation
64 similar manner
65 specificity
66 stimulation
67 suppressor T cells
68 target antigen
69 ultimate goal
70 virus
71 schema:name REGULATION OF HUMAN CYTOLYTIC T LYMPHOCYTES
72 schema:recipient N5d47750767ac43179c3c1a762bf94045
73 sg:person.01007001471.61
74 https://www.grid.ac/institutes/grid.65499.37
75 schema:sameAs https://app.dimensions.ai/details/grant/grant.2467307
76 schema:sdDatePublished 2019-03-07T12:13
77 schema:sdLicense https://scigraph.springernature.com/explorer/license/
78 schema:sdPublisher Nf8f0c659f5be41cfae7f560ca794cf2f
79 schema:startDate 1982-07-01T00:00:00Z
80 schema:url http://projectreporter.nih.gov/project_info_description.cfm?aid=3171888
81 sgo:license sg:explorer/license/
82 sgo:sdDataset grants
83 rdf:type schema:MonetaryGrant
84 N3286ce0b115f4e8786cd1a6853fcd8c1 schema:name dimensions_id
85 schema:value 2467307
86 rdf:type schema:PropertyValue
87 N5d47750767ac43179c3c1a762bf94045 schema:member sg:person.01007001471.61
88 schema:roleName PI
89 rdf:type schema:Role
90 N7227c30ae7a94e26bca81d47d781a660 schema:name nih_id
91 schema:value R01CA034129
92 rdf:type schema:PropertyValue
93 Na852e93fdd0d4bc0b1eb29435304eb94 schema:currency USD
94 schema:value 0
95 rdf:type schema:MonetaryAmount
96 Nf8f0c659f5be41cfae7f560ca794cf2f schema:name Springer Nature - SN SciGraph project
97 rdf:type schema:Organization
98 anzsrc-for:2211 schema:inDefinedTermSet anzsrc-for:
99 rdf:type schema:DefinedTerm
100 sg:person.01007001471.61 schema:affiliation https://www.grid.ac/institutes/grid.65499.37
101 schema:familyName BURAKOFF
102 schema:givenName STEVEN J.
103 rdf:type schema:Person
104 https://www.grid.ac/institutes/grid.48336.3a schema:Organization
105 https://www.grid.ac/institutes/grid.65499.37 schema:name DANA-FARBER CANCER INSTITUTE
106 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...