Prognostic Value of Plasma Mitochondrial DNA and Cytochrome C After Cardiac Arrest View Homepage


Ontology type: schema:MedicalStudy     


Clinical Trial Info

YEARS

2018-2020

ABSTRACT

The aim of the study is to determine prognostic value of plasma mitochondrial DNA and cytochrome C after cardiac arrest. The study will be conducted in three parts: 1. Determine plasma concentrations of mitochondrial DNA and cytochrome C in healthy population. 2. Determine release profile of mitochondrial DNA and cytochrome C to plasma after cardiac arrest. 3. Determine plasma prognostic value of mitochondrial DNA and cytochrome C after cardiac arrest and compare it with established prognostic methods. Detailed Description Scientific background Cardiac arrest is one of leading causes of mortality in developed world. Survival ranges between 15 and 22%. Patients surviving cardiac arrest can have significant neurological impairment. None of currently available diagnostic methods can detect neurological consequences in early post resuscitation period. Biomarkers (NSE - neuron specific enolase, protein S100, GFAP - glial fibrillary acidic protein), imaging (computer tomography, magnetic resonance imaging) and functional studies (EEG - electro encephalography, SSEP - somatosensory evoked potentials) have all shown only limited prognostic value in predicting survival with good neurological outcome after cardiac arrest. Mitochondrial damage is one of key mechanisms of postresuscitation dysfunction. Elevated values of mitochondrial damage-associated molecular patterns were already linked to worst survival after cardiac arrest and critical illness. Mitochondrial damage often results in cell death and mitochondrial damage-associated molecular patterns are released into bloodstream. Mitochondrial damage-associated molecular patterns that can be detected in serum or plasma are: mitochondrial DNA, mitochondrial transcription factor A, N-formyl peptides, succinate, cardiolipin, cytochrome C... With a more sensitive method of early neuroprognostication after cardiac arrest the limited medical resources could be used more effectively in patients with chance of good neurological recovery. Aim of the study The aim of this study is to research the role of mitochondrial damage-associated molecular patterns in patients after cardiac arrest. 1. Determine normal plasma values of mitochondrial damage-associated molecular patterns in healthy population. 2. Compare current prognostic procedures of post-resuscitation neurological damage with prognostic value of mitochondrial DNA and cytochrome C in plasma. Expected results Due to central role of mitochondria in hypoxic-ischemic tissue damage a greater mitochondrial damage (measured trough release of mitochondrial damage-associated molecular patterns) is expected to have direct correlation with extent of tissue damage (also neurological). Currently published data indicate that patients with higher plasma mitochondrial DNA levels after cardiac arrest have higher mortality. Correlation of mitochondrial damage-associated molecular patterns to extent of neurological damage in survivors of cardiac arrest was not researched yet. Methods 1. Measurement of measure mitochondrial damage-associated molecular patterns in healthy population (mitochondrial DNA and cytochrome C). 2. Determination of releasing profile of mitochondrial DNA and cytochrome C in survivors of cardiac arrest and establishment of best sample collection time. 3. Calculation of predictive value for survival of cardiac arrest with good neurological outcome for mitochondrial DNA and cytochrome C. Mitochondrial damage-associated molecular patterns will be measured in samples of plasma. Mitochondrial DNA will be measured using PCR method. Cytochrome C will be measured using ELISA. More... »

URL

https://clinicaltrials.gov/show/NCT03539497

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/3053", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "type": "DefinedTerm"
      }
    ], 
    "description": "The aim of the study is to determine prognostic value of plasma mitochondrial DNA and cytochrome C after cardiac arrest. The study will be conducted in three parts: 1. Determine plasma concentrations of mitochondrial DNA and cytochrome C in healthy population. 2. Determine release profile of mitochondrial DNA and cytochrome C to plasma after cardiac arrest. 3. Determine plasma prognostic value of mitochondrial DNA and cytochrome C after cardiac arrest and compare it with established prognostic methods.\n\nDetailed Description\nScientific background Cardiac arrest is one of leading causes of mortality in developed world. Survival ranges between 15 and 22%. Patients surviving cardiac arrest can have significant neurological impairment. None of currently available diagnostic methods can detect neurological consequences in early post resuscitation period. Biomarkers (NSE - neuron specific enolase, protein S100, GFAP - glial fibrillary acidic protein), imaging (computer tomography, magnetic resonance imaging) and functional studies (EEG - electro encephalography, SSEP - somatosensory evoked potentials) have all shown only limited prognostic value in predicting survival with good neurological outcome after cardiac arrest. Mitochondrial damage is one of key mechanisms of postresuscitation dysfunction. Elevated values of mitochondrial damage-associated molecular patterns were already linked to worst survival after cardiac arrest and critical illness. Mitochondrial damage often results in cell death and mitochondrial damage-associated molecular patterns are released into bloodstream. Mitochondrial damage-associated molecular patterns that can be detected in serum or plasma are: mitochondrial DNA, mitochondrial transcription factor A, N-formyl peptides, succinate, cardiolipin, cytochrome C... With a more sensitive method of early neuroprognostication after cardiac arrest the limited medical resources could be used more effectively in patients with chance of good neurological recovery. Aim of the study The aim of this study is to research the role of mitochondrial damage-associated molecular patterns in patients after cardiac arrest. 1. Determine normal plasma values of mitochondrial damage-associated molecular patterns in healthy population. 2. Compare current prognostic procedures of post-resuscitation neurological damage with prognostic value of mitochondrial DNA and cytochrome C in plasma. Expected results Due to central role of mitochondria in hypoxic-ischemic tissue damage a greater mitochondrial damage (measured trough release of mitochondrial damage-associated molecular patterns) is expected to have direct correlation with extent of tissue damage (also neurological). Currently published data indicate that patients with higher plasma mitochondrial DNA levels after cardiac arrest have higher mortality. Correlation of mitochondrial damage-associated molecular patterns to extent of neurological damage in survivors of cardiac arrest was not researched yet. Methods 1. Measurement of measure mitochondrial damage-associated molecular patterns in healthy population (mitochondrial DNA and cytochrome C). 2. Determination of releasing profile of mitochondrial DNA and cytochrome C in survivors of cardiac arrest and establishment of best sample collection time. 3. Calculation of predictive value for survival of cardiac arrest with good neurological outcome for mitochondrial DNA and cytochrome C. Mitochondrial damage-associated molecular patterns will be measured in samples of plasma. Mitochondrial DNA will be measured using PCR method. Cytochrome C will be measured using ELISA.", 
    "endDate": "2020-06-01T00:00:00Z", 
    "id": "sg:clinicaltrial.NCT03539497", 
    "keywords": [
      "prognostic value", 
      "mitochondrial DNA", 
      "Heart Arrest", 
      "cytochrome", 
      "plasma concentration", 
      "healthy population", 
      "release profile", 
      "plasma", 
      "prognostic", 
      "scientific background", 
      "mortality", 
      "developed world", 
      "survival", 
      "patient", 
      "neurological impairment", 
      "diagnostic method", 
      "neurological consequence", 
      "resuscitation", 
      "Biological Marker", 
      "NSE", 
      "Phosphopyruvate Hydratase", 
      "protein", 
      "GFAP", 
      "glial fibrillary acidic protein", 
      "imaging", 
      "computer tomography", 
      "Magnetic Resonance Imaging", 
      "Electroencephalography", 
      "somatosensory evoked potential", 
      "neurological outcome", 
      "mitochondrial damage", 
      "key mechanism", 
      "dysfunction", 
      "damage-associated molecular pattern", 
      "bad survival", 
      "critical illness", 
      "cell death", 
      "bloodstream", 
      "serum", 
      "mitochondrial transcription", 
      "peptide", 
      "Succinic Acid", 
      "cardiolipin", 
      "sensitive method", 
      "medical resource", 
      "neurological recovery", 
      "normal plasma", 
      "neurological damage", 
      "expected result", 
      "central role", 
      "mitochondrion", 
      "ischemic tissue", 
      "release", 
      "direct correlation", 
      "tissue damage", 
      "published data", 
      "high mortality", 
      "correlation", 
      "survivor", 
      "method 1", 
      "measurement", 
      "determination", 
      "profile", 
      "establishment", 
      "sample collection", 
      "calculation", 
      "predictive value", 
      "sample", 
      "PCR method", 
      "Enzyme-Linked Immunosorbent Assay"
    ], 
    "name": "Prognostic Value of Plasma Mitochondrial DNA and Cytochrome C After Cardiac Arrest", 
    "sameAs": [
      "https://app.dimensions.ai/details/clinical_trial/NCT03539497"
    ], 
    "sdDataset": "clinical_trials", 
    "sdDatePublished": "2019-03-07T15:27", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "file:///pack/app/us_ct_data_00027.json", 
    "sponsor": [
      {
        "id": "https://www.grid.ac/institutes/grid.8954.0", 
        "type": "Organization"
      }, 
      {
        "id": "https://www.grid.ac/institutes/grid.29524.38", 
        "type": "Organization"
      }
    ], 
    "startDate": "2018-11-01T00:00:00Z", 
    "subjectOf": [
      {
        "id": "https://doi.org/10.1016/j.resuscitation.2012.03.032", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012416819"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1136/heartjnl-2015-307450", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015345635"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrneurol.2014.36", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015663821", 
          "https://doi.org/10.1038/nrneurol.2014.36"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.5935/0103-507x.20140043", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019189912"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/ars.2015.6407", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059232568"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "type": "MedicalStudy", 
    "url": "https://clinicaltrials.gov/show/NCT03539497"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/clinicaltrial.NCT03539497'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/clinicaltrial.NCT03539497'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/clinicaltrial.NCT03539497'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/clinicaltrial.NCT03539497'


 

This table displays all metadata directly associated to this object as RDF triples.

107 TRIPLES      16 PREDICATES      91 URIs      79 LITERALS      1 BLANK NODES

Subject Predicate Object
1 sg:clinicaltrial.NCT03539497 schema:about anzsrc-for:3053
2 schema:description The aim of the study is to determine prognostic value of plasma mitochondrial DNA and cytochrome C after cardiac arrest. The study will be conducted in three parts: 1. Determine plasma concentrations of mitochondrial DNA and cytochrome C in healthy population. 2. Determine release profile of mitochondrial DNA and cytochrome C to plasma after cardiac arrest. 3. Determine plasma prognostic value of mitochondrial DNA and cytochrome C after cardiac arrest and compare it with established prognostic methods. Detailed Description Scientific background Cardiac arrest is one of leading causes of mortality in developed world. Survival ranges between 15 and 22%. Patients surviving cardiac arrest can have significant neurological impairment. None of currently available diagnostic methods can detect neurological consequences in early post resuscitation period. Biomarkers (NSE - neuron specific enolase, protein S100, GFAP - glial fibrillary acidic protein), imaging (computer tomography, magnetic resonance imaging) and functional studies (EEG - electro encephalography, SSEP - somatosensory evoked potentials) have all shown only limited prognostic value in predicting survival with good neurological outcome after cardiac arrest. Mitochondrial damage is one of key mechanisms of postresuscitation dysfunction. Elevated values of mitochondrial damage-associated molecular patterns were already linked to worst survival after cardiac arrest and critical illness. Mitochondrial damage often results in cell death and mitochondrial damage-associated molecular patterns are released into bloodstream. Mitochondrial damage-associated molecular patterns that can be detected in serum or plasma are: mitochondrial DNA, mitochondrial transcription factor A, N-formyl peptides, succinate, cardiolipin, cytochrome C... With a more sensitive method of early neuroprognostication after cardiac arrest the limited medical resources could be used more effectively in patients with chance of good neurological recovery. Aim of the study The aim of this study is to research the role of mitochondrial damage-associated molecular patterns in patients after cardiac arrest. 1. Determine normal plasma values of mitochondrial damage-associated molecular patterns in healthy population. 2. Compare current prognostic procedures of post-resuscitation neurological damage with prognostic value of mitochondrial DNA and cytochrome C in plasma. Expected results Due to central role of mitochondria in hypoxic-ischemic tissue damage a greater mitochondrial damage (measured trough release of mitochondrial damage-associated molecular patterns) is expected to have direct correlation with extent of tissue damage (also neurological). Currently published data indicate that patients with higher plasma mitochondrial DNA levels after cardiac arrest have higher mortality. Correlation of mitochondrial damage-associated molecular patterns to extent of neurological damage in survivors of cardiac arrest was not researched yet. Methods 1. Measurement of measure mitochondrial damage-associated molecular patterns in healthy population (mitochondrial DNA and cytochrome C). 2. Determination of releasing profile of mitochondrial DNA and cytochrome C in survivors of cardiac arrest and establishment of best sample collection time. 3. Calculation of predictive value for survival of cardiac arrest with good neurological outcome for mitochondrial DNA and cytochrome C. Mitochondrial damage-associated molecular patterns will be measured in samples of plasma. Mitochondrial DNA will be measured using PCR method. Cytochrome C will be measured using ELISA.
3 schema:endDate 2020-06-01T00:00:00Z
4 schema:keywords Biological Marker
5 Electroencephalography
6 Enzyme-Linked Immunosorbent Assay
7 GFAP
8 Heart Arrest
9 Magnetic Resonance Imaging
10 NSE
11 PCR method
12 Phosphopyruvate Hydratase
13 Succinic Acid
14 bad survival
15 bloodstream
16 calculation
17 cardiolipin
18 cell death
19 central role
20 computer tomography
21 correlation
22 critical illness
23 cytochrome
24 damage-associated molecular pattern
25 determination
26 developed world
27 diagnostic method
28 direct correlation
29 dysfunction
30 establishment
31 expected result
32 glial fibrillary acidic protein
33 healthy population
34 high mortality
35 imaging
36 ischemic tissue
37 key mechanism
38 measurement
39 medical resource
40 method 1
41 mitochondrial DNA
42 mitochondrial damage
43 mitochondrial transcription
44 mitochondrion
45 mortality
46 neurological consequence
47 neurological damage
48 neurological impairment
49 neurological outcome
50 neurological recovery
51 normal plasma
52 patient
53 peptide
54 plasma
55 plasma concentration
56 predictive value
57 profile
58 prognostic
59 prognostic value
60 protein
61 published data
62 release
63 release profile
64 resuscitation
65 sample
66 sample collection
67 scientific background
68 sensitive method
69 serum
70 somatosensory evoked potential
71 survival
72 survivor
73 tissue damage
74 schema:name Prognostic Value of Plasma Mitochondrial DNA and Cytochrome C After Cardiac Arrest
75 schema:sameAs https://app.dimensions.ai/details/clinical_trial/NCT03539497
76 schema:sdDatePublished 2019-03-07T15:27
77 schema:sdLicense https://scigraph.springernature.com/explorer/license/
78 schema:sdPublisher Na78bfcf2c3634a3badb1cb120d485090
79 schema:sponsor https://www.grid.ac/institutes/grid.29524.38
80 https://www.grid.ac/institutes/grid.8954.0
81 schema:startDate 2018-11-01T00:00:00Z
82 schema:subjectOf sg:pub.10.1038/nrneurol.2014.36
83 https://doi.org/10.1016/j.resuscitation.2012.03.032
84 https://doi.org/10.1089/ars.2015.6407
85 https://doi.org/10.1136/heartjnl-2015-307450
86 https://doi.org/10.5935/0103-507x.20140043
87 schema:url https://clinicaltrials.gov/show/NCT03539497
88 sgo:license sg:explorer/license/
89 sgo:sdDataset clinical_trials
90 rdf:type schema:MedicalStudy
91 Na78bfcf2c3634a3badb1cb120d485090 schema:name Springer Nature - SN SciGraph project
92 rdf:type schema:Organization
93 anzsrc-for:3053 schema:inDefinedTermSet anzsrc-for:
94 rdf:type schema:DefinedTerm
95 sg:pub.10.1038/nrneurol.2014.36 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015663821
96 https://doi.org/10.1038/nrneurol.2014.36
97 rdf:type schema:CreativeWork
98 https://doi.org/10.1016/j.resuscitation.2012.03.032 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012416819
99 rdf:type schema:CreativeWork
100 https://doi.org/10.1089/ars.2015.6407 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059232568
101 rdf:type schema:CreativeWork
102 https://doi.org/10.1136/heartjnl-2015-307450 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015345635
103 rdf:type schema:CreativeWork
104 https://doi.org/10.5935/0103-507x.20140043 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019189912
105 rdf:type schema:CreativeWork
106 https://www.grid.ac/institutes/grid.29524.38 schema:Organization
107 https://www.grid.ac/institutes/grid.8954.0 schema:Organization
 




Preview window. Press ESC to close (or click here)


...